Valorization of soy whey wastewater: How epigallocatechin-3-gallate regulates protein precipitation
Valorization of soy whey wastewater: How epigallocatechin-3-gallate regulates protein precipitation
复制标题
大豆乳清废水的增值：表没食子儿茶素-3-没食子酸酯如何调节蛋白质沉淀
DOI：
10.1021/acssuschemeng.9b03208
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发表时间：
2019
影响因子：
8.4
通讯作者：
Sui Xiaonan
中科院分区：
文献类型：
--
作者：
Xu Zejian;Hao Nairong;Li Lianwei;Zhang Yan;Yu Liangli;Jiang Lianzhou;Sui Xiaonan
大豆基食品的生产会产生大量的大豆乳清废水（SWW）形式的废物。由于SWW的高蛋白含量，需要对其进行增值。为此，本研究旨在开发一种可持续的和环境友好的策略，通过使用表没食子儿茶素-3-没食子酸酯（EGCG）沉淀蛋白质从SWW中回收大豆蛋白。的沉淀过程进行了优化，相对于样品的pH值和所使用的EGCG的量，和蛋白质-EGCG聚集体的特征在于，以获得其结构和形态学特性的见解。表没食子儿茶素没食子酸酯（EGCG）调节SWW中的蛋白质形成具有3D硬球构型的蛋白质-表没食子儿茶素没食子酸酯（EGCG）聚集体。此外，形成的蛋白质-EGCG聚集体具有比其蛋白质前体大得多的分子量和尺寸。这种有趣的现象被描述为蛋白质“雪球”亚基组装成更大的蛋白质-EGCG聚集体。本研究结果表明，高蛋白回收率的...
The manufacture of soy-based foods generates significant wastes in the form of soy whey wastewater (SWW). There is a demand for the valorization of SWW due to its high protein content. For this purpose, this study aims at developing a sustainable and environmentally friendly strategy in the recovery of soy proteins from SWW through precipitation proteins using epigallocatechin-3-gallate (EGCG). The precipitation process was optimized with respect to the sample pH and amount of EGCG used, and protein–EGCG aggregates were characterized to obtain insights on their structural and morphological properties. EGCG regulates proteins in SWW to form protein–EGCG aggregates with a 3D hard-sphere configuration. Also, the formed protein–EGCG aggregates have a much greater molecular weight and size than their protein precursor. This interesting phenomenon was depicted as the assembly of protein “snowball” subunits into larger protein–EGCG aggregates. Results from the present study showed that a high protein recovery of...