EphA2/EphrinA1 mRNA Expression and Protein Production in Adenoid Cystic Carcinoma of Salivary Gland
EphA2/EphrinA1 mRNA Expression and Protein Production in Adenoid Cystic Carcinoma of Salivary Gland
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唾液腺腺样囊性癌中 EphA2/ephrinA1 mRNA 表达和蛋白产生。
DOI：
10.1016/j.joms.2012.10.026
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发表时间：
2013-05-01
影响因子：
1.9
通讯作者：
Shang, ZhengJun
中科院分区：
文献类型：
--
作者：
Shao, Zhe;Zhu, Fei;Shang, ZhengJun
目的：EphA2/ephrinA1被认为在肿瘤生长和转移中起作用。本研究的目的是确定EphA2/ephrinA1在腺样囊性癌的信使核糖核酸（mRNA）和蛋白质中的存在情况。
材料和方法：采用实时逆转录聚合酶链反应、蛋白质印迹法和免疫组织化学方法研究腺样囊性癌中EphA2和ephrinA1的mRNA及蛋白质表达以及蛋白质产物。还研究了腺样囊性癌细胞中EphA2的酪氨酸磷酸化状态。
结果：在腺样囊性癌组织中检测到EphA2和ephrinA1蛋白质及mRNA的高表达。腺样囊性癌中EphA2/ephrinA1的染色活性比正常腺体组织更显著（P <.01）。EphA2/ephrinA1的表达与微血管密度显著相关（P <.01）。EphA2/ephrinA1的表达和微血管密度与临床TNM分期、神经周围侵犯和血管侵犯相关（P <.05）。在腺样囊性癌的3种组织学类型中，实体型中EphA2/ephrinA1的表达和微血管密度明显高于筛状型和管状型（P <.01）。我们还注意到EphA2以非酪氨酸磷酸化状态存在。
结论：本研究表明EphA2/ephrinA1在腺样囊性癌中高表达。EphA2/ephrinA1可作为腺样囊性癌的一种新的治疗靶点。（C）2013年美国口腔颌面外科医师协会《口腔颌面外科杂志》2013年71卷869 - 878页
Purpose: EphA2/ephrinA1 is believed to play a role in tumor growth and metastasis. The purpose of the present study was to determine the presence of EphA2/ephrinA1 in mRNA and protein adenoid cystic carcinoma.Materials and Methods: mRNA and protein expression and protein product of EphA2 and ephrinA1 in adenoid cystic carcinoma was investigated using real-time reverse transcriptase polymerase chain reaction, Western blot, and immunohistochemistry. The tyrosine-phosphorylated state of EphA2 in adenoid cystic carcinoma cells was also investigated.Results: Greater expression of EphA2 and ephrinA1 proteins and mRNA was detected in adenoid cystic carcinoma tissues. EphA2/ephrinA1 staining activities in adenoid cystic carcinoma were more significant than those in normal gland tissue (P < .01). EphA2/ephrinA1 expression correlated significantly to the microvessel density (P < .01). EphA2/ephrinA1 expression and microvessel density correlated with the clinical TNM stage, perineural invasion, and vascular invasion (P < .05). In 3 histologic types of adenoid cystic carcinoma, the expression of EphA2/ephrinA1 and microvessel density was significantly greater in the solid type than in the cribriform and tubular types (P < .01). We also noted that EphA2 was present in a nontyrosine-phosphorylated state.Conclusions: The present study showed a high expression of EphA2/ephrinA1 in adenoid cystic carcinoma. EphA2/ephrinA1 can serve as a novel therapy target for adenoid cystic carcinoma. (C) 2013 American Association of Oral and Maxillofacial Surgeons J Oral Maxillofac Surg 71:869-878, 2013