Occurrence of periodontal bacteria in healthy children: a 2-year longitudinal study
Occurrence of periodontal bacteria in healthy children: a 2-year longitudinal study
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DOI：
10.1046/j.1600-0528.2003.00112.x
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发表时间：
2003-12-01
影响因子：
2.3
通讯作者：
Kimura, S
中科院分区：
文献类型：
--
作者：
Ooshima, T;Nishiyama, N;Kimura, S
目的：了解儿童青少年牙周特殊细菌的发生情况。方法：采用聚合酶链式反应方法，对119名2~15岁牙周健康儿童的菌斑和唾液标本进行纵向检测。结果：唾液中常见胞噬杆菌、棕黄色葡萄球菌、伴生放线杆菌，且在接下来的研究中仍在唾液中持续存在，而牙龈卟啉单胞菌、齿密螺旋体和中间普氏杆菌很少检出。斑块中黑色假单胞菌阳性率较高，且患病率随年龄增长而增加。在菌斑和唾液中有时检测到腐蚀性艾肯氏杆菌和直肠弯曲杆菌，在唾液中偶尔检测到福氏坦纳氏菌。结论：伴生放线菌、白色念珠菌、芽孢杆菌、黑色假单胞菌、直链球菌和腐蚀性假单胞菌是健康儿童口腔微生物区系的常见成员，而牙龈假单胞菌、中间假单胞菌和齿假单胞菌似乎是暂时性微生物。
Objectives: To examine the occurrence of specific periodontal bacteria in children and adolescents. Methods:Ten putative periodontal bacteria were longitudinally examined in plaque and saliva samples from 119 periodontally healthy children (2-15 years old) using a polymerase chain reaction method. Results: Capnocytophaga ochracea, C. sputigena, and Actinobacillus actinomycetemcomitans were frequently found in saliva, and tended to persist in saliva for the remainder of the study, whereas Porphyromonas gingivalis, Treponema denticola, and Prevotella intermedia were rarely detected. P. nigrescens was more frequently detected in plaque and its prevalence increased with age. Eikenella corrodens and Campylobacter rectus were sometimes detected in both plaque and saliva, while Tannerella forsythensis was occasionally detected in saliva. Conclusions: A. actinomycetemcomitans, C. ochracea, C. sputigena, P. nigrescens, C. rectus, and E. corrodens are common members of the oral microbial flora of healthy children, whereas P. gingivalis, P. intermedia, and T. denticola appear to be transient organisms.