Electrical stimulation of the rostral medial prefrontal cortex in rabbits inhibits the expression of conditioned eyelid responses but not their acquisition
Electrical stimulation of the rostral medial prefrontal cortex in rabbits inhibits the expression of conditioned eyelid responses but not their acquisition
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DOI：
10.1073/pnas.0704548104
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发表时间：
2007-07-03
影响因子：
11.1
通讯作者：
Gruart, Agnes
中科院分区：
文献类型：
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作者：
Leal-Campanario, Rocio;Fairen, Alfonso;Gruart, Agnes
我们研究了嘴内侧前额叶皮质(MPFC)在警觉兔的反射性眨眼和经典条件眼皮反应中的作用。从丘脑内侧背侧核复合体的内侧半侧发出的传入投射来识别吻侧的mPFC。经典的条件反射包括以370ms的音调作为条件刺激(CS)和以100ms的空气喷射左侧角膜作为非条件刺激(US)的延迟模式。CS与美国联手终止。电刺激对侧头侧mPFC可显著抑制喷气诱发的眨眼反应。与对照组动物相比，在CS-US间期连续10次对嘴侧mPFC进行相同的训练刺激，显著减少了条件性反应(CRS)的产生。有趣的是，从第五次条件化训练开始，去掉对嘴侧mPFC的训练刺激，CRS的百分比几乎达到了控制值。电刺激状态良好的动物的肩部mPFC，可使CRS的百分比显著降低。此外，刺激头端mPFC还能改变诱发CRS的运动学(潜伏期、波幅和速度)。这些结果表明，嘴侧mPFC是反射性诱发和经典条件眨眼的有效抑制物，但激活只阻止CRS的表达，而不是它们的潜在习得。讨论了吻侧mPFC这种抑制作用的功能和行为意义。
We have studied the role of rostral medial prefrontal cortex (mPFC) on reflexively evoked blinks and on classically conditioned eyelid responses in alert-behaving rabbits. The rostral mPFC was identified by its afferent projections from the medial half of the thalamic mediodorsal nuclear complex. Classical conditioning consisted of a delay paradigm using a 370-ms tone as the conditioned stimulus (CS) and a 100-ms air puff directed at the left cornea as the unconditioned stimulus (US). The CS coterminated with the US. Electrical train stimulation of the contralateral rostral mPFC produced a significant inhibition of air-puff-evoked blinks. The same train stimulation of the rostral mPFC presented during the CS-US interval for 10 successive conditioning sessions significantly reduced the generation of conditioned responses (CRs) as compared with values reached by control animals. Interestingly, the percentage of CRs almost reached control values when train stimulation of the rostral mPFC was removed from the fifth conditioning session on. The electrical stimulation of the rostral mPFC in well conditioned animals produced a significant decrease in the percentage of CRs. Moreover, the stimulation of the rostral mPFC was also able to modify the kinematics (latency, amplitude, and velocity) of evoked CRs. These results suggest that the rostral mPFC is a potent inhibitor of reflexively evoked and classically conditioned eye-blinks but that activation prevents only the expression of CRs, not their latent acquisition. Functional and behavioral implications of this inhibitory role of the rostral mPFC are discussed.