IDENTIFICATION OF POLIOVIRUS PARTICLES OF DIFFERENT ANTIGENICITY BY SPECIFIC AGGLUTINATION AS SEEN IN ELECTRON MICROSCOPE
IDENTIFICATION OF POLIOVIRUS PARTICLES OF DIFFERENT ANTIGENICITY BY SPECIFIC AGGLUTINATION AS SEEN IN ELECTRON MICROSCOPE
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DOI：
10.1016/0042-6822(62)90205-2
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发表时间：
1962-01-01
期刊：
影响因子：
3.7
通讯作者：
ANDERSON, TF
中科院分区：
文献类型：
--
作者：
HUMMELER, K;BROWN, RA;ANDERSON, TF
1型脊髓灰质炎病毒颗粒凝集的类型特异性抗血清和聚集体进行了研究，电子显微镜下的磷钨酸包埋后，其组成。使用单特异性抗血清的N或H抗原，它被发现，抗N引起凝集的基本上是“完整的”颗粒，而抗H产生的聚集体的明显缺陷的颗粒。同时添加抗-N和抗-H，如兔血清中所含，病毒制剂，导致形成混合的聚集体，而连续添加的单特异性抗血清分离的抗原成分成基本上特定的聚集体。同时和连续添加抗-N和抗-H中观察到的差异的可能原因进行了讨论。
Poliovirus particles of type 1 were agglutinated by type-specific antisera and the aggregates were studied electron microscopically as to their composition after embedding in phosphotungstic acid. Using monospecific antisera to either the N or H antigens it was found that anti-N caused agglutination of essentially "complete" particles, whereas anti-H produced aggregates of obviously defective particles. Simultaneous addition of anti-N and anti-H, as contained in a rabbit serum, to the virus preparation, resulted in formation of mixed aggregates, whereas successive addition of monospecific antisera separated the antigenic components into essentially specific aggregates. Possible reasons for the differences observed in simultaneous and successive addition of anti-N and anti-H have been discussed.