Differential resistance of insect sodium channels with kdr mutations to deltamethrin, permethrin and DDT
Differential resistance of insect sodium channels with kdr mutations to deltamethrin, permethrin and DDT
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DOI：
10.1016/j.ibmb.2011.05.004
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发表时间：
2011-09-01
影响因子：
3.8
通讯作者：
Williamson, Martin S.
中科院分区：
文献类型：
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作者：
Burton, Mark J.;Mellor, Ian R.;Williamson, Martin S.
昆虫的击倒抗性（kdr）是由电压门控钠通道（VGSC）基因中遗传性核苷酸多态性引起的，是拟除虫菊酯杀虫剂功效的主要威胁。 VGSC 中 L1014F 替代产生的经典 kdr 现在存在于许多害虫物种中。 L1014 基因座上的另外两个取代也已报道，即 L1014S 和 L1014H。在这里，我们使用 L1014 修饰的果蝇 para VGSC 在异种脓卵母细胞中的表达，并使用两电极电压钳来表征所有三种突变。突变L1014F和L1014H引起半激活电压（V（50，act））显着去极化变化，分别从-17.3 mV（野生型）到-13.1和-13.5 mV，而L1014S没有引起V（50，act）变化，但其电流衰减速度明显快于野生型通道。用溴氰菊酯 (>= 1 nM)、氯菊酯 (>= 30 nM) 或 DDT (>= 1 μM) 处理野生型通道导致 V(50,act) 的超极化转变。溴氰菊酯、氯菊酯和DDT也产生“尾流”，EC(50)分别为0.043、0.40和65μM，最大修饰分别为837、325和7%。 L1014F 的两个测量参数均对所有杀虫剂具有高水平的抗性。 L1014H 最有效地对抗溴氰菊酯引起的尾电流，而 L1014S 则强烈抵抗 DDT 引起的 V(50,act) 的大变化。我们得出的结论是，L1014H 和 L1014S 可能分别是通过大量接触特定拟除虫菊酯和滴滴涕而产生的。 (C) 2011 Elsevier Ltd. 保留所有权利。
Knockdown resistance (kdr) in insects, caused by inherited nucleotide polymorphisms in the voltage-gated sodium channel (VGSC) gene, is a major threat to the efficacy of pyrethroid insecticides. Classic kdr, resulting from an L1014F substitution in the VGSC is now present in numerous pest species. Two other substitutions at the L1014 locus have also been reported, L1014S and L1014H. Here we have used expression of L1014 modified Drosophila para VGSCs in Xeno pus oocytes with two-electrode voltage clamp to characterise all three mutations. The mutations L1014F and L1014H caused significant depolarizing shifts in the half activation voltage (V(50,act)) from -17.3 mV (wild-type) to -13.1 and -13.5 mV respectively, whereas L1014S caused no shift in V(50,act) but its currents decayed significantly faster than wild-type channels. Treatment of the wild-type channel with deltamethrin (>= 1 nM), permethrin (>= 30 nM) or DDT (>= 1 mu M) resulted in hyperpolarizing shifts in V(50,act). Deltamethrin, permethrin and DDT also produced "tail currents" with EC(50)s of 0.043, 0.40 and 65 mu M and maximum modifications of 837, 325 and 7% respectively. L1014F provided a high level of resistance against all insecticides for both measured parameters. L1014H most effectively combated deltamethrin induced tail currents while L1014S strongly resisted the large DDT induced shifts in V(50,act). We conclude that L1014H and L1014S may have arisen through heavy exposure to specific pyrethroids and DDT respectively. (C) 2011 Elsevier Ltd. All rights reserved.