Isolation and characterisation of cancer stem cells from canine osteosarcoma
Isolation and characterisation of cancer stem cells from canine osteosarcoma
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DOI：
10.1016/j.tvjl.2007.07.025
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发表时间：
2008-01-01
影响因子：
2.2
通讯作者：
Argyle, D. J.
中科院分区：
文献类型：
--
作者：
Wilson, H.;Huelsmeyer, M.;Argyle, D. J.
越来越多的证据表明，癌症是一种干细胞疾病。这项研究试图从犬骨肉瘤中分离和鉴定癌症干细胞。1个人细胞系和3个犬细胞系在非贴壁培养条件下用血清饥饿的半固体培养液培养。在这些条件下鉴定了所有细胞系的原始南极集落，并利用胚胎干细胞标记的分子和细胞化学技术对其进行了表征。胚胎干细胞相关基因Nanog、Oct4和STAT3的表达表明其为原始表型。经多次传代后，菌丝球可稳定繁殖，恢复正常生长条件时可产生贴壁细胞培养物。人类和狗的骨肉瘤细胞系之间的相似性增加了狗作为人类疾病模型的潜力。(C)2007爱思唯尔有限公司。保留所有权利。
There is increasing evidence that cancer is a stem cell disease. This study sought to isolate and characterise cancer stem cells from canine osteosarcoma. One human and three canine cell lines were cultured in non-adherent culture conditions using serum-starved, semi-solid media. Primitive sarcosphere colonies from all cell lines were identified under these conditions and were characterised using molecular and cytochemical techniques for embryonic stem cell markers. Expression of the embryonic stem cell-associated genes Nanog, Oct4 and STAT3 indicated a primitive phenotype. Sarcospheres could be reproduced consistently when passaged multiple times and produced adherent cell cultures when returned to normal growth conditions. Similarities between human and canine osteosarcoma cell lines add credence to the potential of the dog as a model for human disease. (C) 2007 Elsevier Ltd. All rights reserved.