Structural and functional characterization of peptide-beta2m fused HLA-A2/MART1(27-35) complexes.
Structural and functional characterization of peptide-beta2m fused HLA-A2/MART1(27-35) complexes.
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发表时间：
2006
影响因子：
3.1
通讯作者：
Chuanlai Shen;Chien-Chung Chang;Jianqiong Zhang;W. Guo;Lingzhi Xia;F. Meng;W. Xie
中科院分区：
文献类型：
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作者：
Chuanlai Shen;Chien-Chung Chang;Jianqiong Zhang;W. Guo;Lingzhi Xia;F. Meng;W. Xie
利用可溶性的HLAI类/多肽复合体来监测抗原反应性T细胞往往受到低产率和高成本生产的阻碍。作为一种替代策略，多肽-β(2)m融合的2组分(2C)HLAI/多肽复合体已被开发出来，但由于缺乏与传统的3组分(3C)复合体的结构和功能特征的比较，其应用受到限制。在本研究中，我们证明了2C和3C的HLAA2/MART1(27-35)复合体具有相似的层析图谱和相似的稳定性，但前者的产率是后者的2.5倍，并且与HLAA2/MART1(27-35)复合体的特异性受体的结合能力显著高于后者。此外，2C复合体具有类似的刺激特异性CTL增殖的能力，但与其3C复合体相比，在诱导抗原特异性CTL的细胞毒性方面似乎更有效。
The uses of soluble HLA class I/peptide complexes to monitor antigen reactive T cells are often hampered by their low-yield and high-cost production. As an alternative strategy, the peptide-beta(2)m fused, 2-component (2C) HLA class I/peptide complex has been developed, but its application is limited due to the lack of the comparison of its structural and functional characteristics with those of its conventional 3-component (3C) counterpart. In this study, we have demonstrated that the 2C and 3C HLA-A2/MART1(27-35) complexes have a similar chromatographical profile and comparable stability, but the former has 2.5 times higher yield and significantly higher binding ability with HLA-A2/MART1(27-35) complex-specific receptors than the latter. Furthermore, the 2C complex has a comparable ability to stimulate specific CTL proliferation, but appears to be more effective in eliciting the cytotoxicity of antigen-specific CTL, as compared to its 3C counterpart.