Chlorhexidine possesses unique cytotoxic actions in rat thymic lymphocytes: Its relation with electrochemical property of membranes
Chlorhexidine possesses unique cytotoxic actions in rat thymic lymphocytes: Its relation with electrochemical property of membranes
复制标题
氯己定对大鼠胸腺淋巴细胞具有独特的细胞毒作用：其与细胞膜电化学性质的关系
DOI：
10.1016/j.etap.2016.09.024
复制
发表时间：
2016
影响因子：
4.3
通讯作者：
Shiro Ishida and Yasuo Oyama
中科院分区：
文献类型：
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作者：
Kayo Nonami;Shohei Saitoh;Yumiko Nishimura-Danjobara;Shiro Ishida and Yasuo Oyama
氯己定（CHX）是一种用于各种类型药品的抗菌剂。因此，CHX很容易在我们周围找到。由于其带正电荷，细胞膜的电化学性质被认为是CHX细胞毒作用的关键。细胞膜的去极化减弱了CHX对大鼠胸腺淋巴细胞的毒性作用。CHX干扰膜联蛋白V与膜的结合。操纵，以诱导暴露的磷脂酰丝氨酸的外膜表面增强的细胞毒性作用的CHX，表明在膜的电化学性质的变化影响的细胞毒性作用的CHX。因此，CHX可能会杀死生理上经历凋亡的细胞，而不是导致坏死性细胞死亡。然而，该体外研究中的阈值CHX浓度略高于临床观察到的血液CHX浓度。因此，尽管CHX具有本研究中描述的独特细胞毒性作用，但这些结果可能支持CHX使用的安全性。
Chlorhexidine (CHX) is an antibacterial agent used in various types of pharmaceutical products. Therefore, CHX is easily found around us. Owing to its positive charge, the electrochemical property of cell membranes was assumed to be a key point of cytotoxic action of CHX. Depolarization of membranes attenuated the cytotoxic action of CHX in rat thymic lymphocytes. CHX interfered with annexin V binding to membranes. Manipulations to induce exposure of phosphatidylserine on the outer membrane surface augmented the cytotoxic action of CHX, indicating that changes in the electrochemical property of membranes affected the cytotoxic action of CHX. Hence, CHX might kill cells physiologically undergoing apoptosis, resulting instead in necrotic cell death. However, the threshold CHX concentration in this in vitro study was slightly higher than blood CHX concentrations observed clinically. Therefore, these results may support the safety of CHX use although CHX possesses unique cytotoxic actions described in this study.