Impact of UV sterilization and short term storage on the in vitro release kinetics and bioactivity of biomolecules from electrospun scaffolds
Impact of UV sterilization and short term storage on the in vitro release kinetics and bioactivity of biomolecules from electrospun scaffolds
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DOI：
10.1038/s41598-019-51513-1
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发表时间：
2019-10-22
影响因子：
4.6
通讯作者：
Buschmann, Johanna
中科院分区：
文献类型：
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作者：
Evrova, Olivera;Kellenberger, Damian;Buschmann, Johanna
为了有效地将生物活性支架转化为临床前环境，需要仔细考虑适当的灭菌技术和储存条件，因为所选择的灭菌技术和储存条件可能会影响支架的结构和机械性能，以及掺入的生物分子的生物活性和释放动力学。由于很少进行测试或量化，我们在概念验证研究中展示了这些参数如何受到紫外线灭菌和在不同温度下储存一周的影响，使用生物活性静电纺丝DegraPol支架，专门设计用于肌腱断裂修复领域。尽管UV灭菌和不同的储存条件不影响生物活性支架的形态或物理化学性质，但UV灭菌引起生长因子释放动力学的显著衰减，在此血小板衍生生长因子（PDGF-BB）释放（约为100%）。85%）和抗坏血酸释放的轻微降低（约20%）。相比之下，4 ℃和-20 ℃储存对PDGF-BB的释放动力学没有主要影响，而在室温下储存导致释放的PDGF-BB增加。所有储存条件对抗坏血酸释放的影响不大。同样重要的是，UV灭菌和储存均不影响释放的PDGF-BB的生物活性，表明生物活性支架至少稳定一周，并显示生物活性DegraPol支架转化为现成可用产品的潜力。预计这些参数是支架和蛋白质依赖性的。
To effectively translate bioactive scaffolds into a preclinical setting, proper sterilization techniques and storage conditions need to be carefully considered, as the chosen sterilization technique and storage condition might affect the structural and mechanical properties of the scaffolds, as well as the bioactivity and release kinetics of the incorporated biomolecules. Since rarely tested or quantified, we show here in a proof-of-concept study how these parameters are affected by UV sterilization and one week storage at different temperatures using bioactive electrospun DegraPol scaffolds that were specifically designed for application in the field of tendon rupture repair. Even though UV sterilization and the different storage conditions did not impact the morphology or the physicochemical properties of the bioactive scaffolds, UV sterilization caused significant attenuation of the growth factor release kinetics, here platelet derived growth factor (PDGF-BB) release (by approx. 85%) and slight decrease in ascorbic acid release (by approx. 20%). In contrast, 4 degrees C and -20 degrees C storage did not have a major effect on the release kinetics of PDGF-BB, while storage at room temperature caused increase in PDGF-BB released. All storage conditions had little effect on ascorbic acid release. Equally important, neither UV sterilization nor storage affected the bioactivity of the released PDGF-BB, suggesting stability of the bioactive scaffolds for at least one week and showing potential for bioactive DegraPol scaffolds to be translated into an off-the-shelf available product. These parameters are expected to be scaffold and protein-dependent.