KERN CANYON VIRUS - ELECTRON MICROSCOPIC AND IMMUNOLOGICAL STUDIES
KERN CANYON VIRUS - ELECTRON MICROSCOPIC AND IMMUNOLOGICAL STUDIES
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DOI：
10.1016/0042-6822(67)90063-3
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发表时间：
1967-01-01
期刊：
影响因子：
3.7
通讯作者：
FIELDS, BN
中科院分区：
文献类型：
--
作者：
MURPHY, FA;FIELDS, BN
用负对比和电镜观察了在乳鼠脑和细胞培养物中增殖的克恩峡谷病毒（KCV）。该病毒被发现表现出与口腔病毒科的其他病毒相似的对称性。子弹形病毒颗粒的平均长度为132 m [mu]，直径为73 m [mu]。横纹、轴向通道和表面突起突出。病毒成熟发生在边缘细胞质膜上，成熟病毒粒子在细胞外积累。补体结合，病毒中和，和免疫扩散试验比较KCV与其他子弹形病毒的动物证实了不同的抗原性的病毒，而比较KCV与水泡性口炎病毒在密度梯度ultracentrugation实验强调的物理基础，包括KCV在口腔病毒科的病毒。
Kern Canyon virus (KCV), propagated in suckling mouse brain and cell culture, was examined by negative contrast and thin section electron microscopy. The virus was found to exhibit symmetry similar to other viruses of the Stomatoviridae family. The bullet-shaped virus particles had a mean length of 132 m[mu] and were 73 m[mu] in diameter. Cross striations, axial channels and surface projections were prominent. Virus maturation occurred on marginal cytoplasmic membranes with mature virions accumulating extracellularly. Complement fixation, virus neutralization, and immunodiffusion tests comparing KCV with other bullet-shaped viruses of animals confirmed the distinct antigenicity of the virus, whereas comparison of KCV with vesicular stomatitis virus in a density gradient ultracentrifugation experiment emphasized the physical basis for including KCV within the Stomatoviridae family of viruses.