Androgen receptor immunoreactivity in forebrain axons and dendrites in the rat
Androgen receptor immunoreactivity in forebrain axons and dendrites in the rat
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DOI：
10.1210/en.2002-0105
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发表时间：
2003-08-01
期刊：
影响因子：
4.8
通讯作者：
Azcoitia, I
中科院分区：
文献类型：
--
作者：
DonCarlos, LL;Garcia-Ovejero, D;Azcoitia, I
雄激素受体(Ars)是类固醇受体超家族的成员，传统上被认为是转录因子。在配体存在的情况下，AR驻留在细胞核中，在配体结合时，受体二聚化并与激素反应基因启动子区域的特定反应元件结合。然而，在这份报告中，我们描述了一项发现，即ARs也存在于哺乳动物中枢神经系统的轴突和树突中。用针对AR蛋白N端的两种不同抗体和镍强化的3‘-3’-二氨基联胺，以及荧光方法和共聚焦显微镜，在光镜水平上观察了AR在大鼠脑内轴突的表达。这种分布在超微结构水平得到了证实。此外，在超微结构水平上，AR免疫反应在小树突中也被发现。AR免疫反应阳性轴突主要见于大脑皮层，在AR核表达丰富的区域很少见。观察到AR存在于轴突和树突中，突显了雄激素在神经元功能中发挥重要的和新的核外作用的可能性。
As members of the steroid receptor superfamily, androgen receptors (ARs) have been traditionally identified as transcription factors. In the presence of ligand, ARs reside in the nucleus, where, upon ligand binding, the receptors dimerize and bind to specific response elements in the promoter region of hormone-responsive genes. However, in this report, we describe the discovery that ARs are also present in axons and dendrites within the mammalian central nervous system. AR expression in axons was identified in the rat brain at the light microscopic level using two different antibodies directed against the N terminus of the AR protein and nickel intensified 3'-3'-diaminobenzidine, and also using fluorescence methods and confocal microscopy. This distribution was confirmed at the ultrastructural level. In addition, AR immunoreactivity was identified in small dendrites at the ultrastructural level. AR-immunoreactive axons were observed primarily in the cerebral cortex and were rare in regions where nuclear AR expression is abundant. The observation that ARs are present in axons and dendrites highlights the possibility that androgens play an important and novel extranuclear role in neuronal function.