Identification of TIA-1+ and Granzyme B+ Cytotoxic T Cells in Lichen sclerosus et atrophicus
Identification of TIA-1+ and Granzyme B+ Cytotoxic T Cells in Lichen sclerosus et atrophicus
复制标题
硬化性萎缩地衣中 TIA-1 和颗粒酶 B 细胞毒性 T 细胞的鉴定
作者：
T. Gross;A. Wagner;S. Ugurel;W. Tilgen;U. Reinhold
背景资料：皮肤硬化性萎缩性苔藓（LSA）的发病和持续与包括CD 4+和CD 8+细胞在内的CD 3 + T细胞的炎性浸润有关。这些细胞存在的功能相关性尚不清楚。目的：本研究旨在定量LSA病变中静息和活化的细胞毒性T细胞。方法：对20例活动期LSA患者进行研究。皮肤浸润性T细胞的免疫组织学特征与抗CD 3，CD 8，T细胞限制性细胞内抗原（TIA-1）和颗粒酶B（GrB）的抗体。TIA-1标记静息和活化T细胞的细胞毒性颗粒，而GrB表示活化的细胞毒性T淋巴细胞（CTL）。结果：在所有病例中，一致发现大量T细胞表达细胞毒性颗粒。结果表明大量浸润的CD 8 + TIA+ T细胞。此外，一个显着的数量GrB+激活的CTL与水肿变性的基底细胞层中发现真皮浸润和真皮表皮界面。结论：LSA中高比例的皮肤浸润性T细胞具有潜在的细胞毒作用。结果表明，基底角质形成细胞的水肿变性可能至少部分介导的CTL依赖性机制。我们的数据还表明，细胞介导的免疫反应可能在疾病的发病机制中发挥重要作用。
Background: The onset and persistence of cutaneous lichen sclerosus et atrophicus (LSA) are linked to the presence of an inflammatory infiltrate of CD3+ T cells that includes CD4+ and CD8+ cells. The functional relevance of the presence of these cells is unknown. Objective: The study intended to quantify resting and activated cytotoxic T cells in LSA lesions. Methods: Twenty patients with active LSA were studied. Skin-infiltrating T cells were immunohistologically characterized with antibodies against CD3, CD8, T-cell-restricted intracellular antigen (TIA-1) and granzyme B (GrB). TIA-1 labels cytotoxic granules of resting and activated T cells, whereas GrB designates activated cytotoxic T lymphocytes (CTL). Results: In all cases, numerous T cells were consistently found expressing cytotoxic granules. The results indicated a high number of infiltrating CD8+ TIA+ T cells. Furthermore, a notable number of GrB+ activated CTL associated with hydropic degeneration of the basal cell layer were found within the dermal infiltrate and at the dermoepidermal interface. Conclusion: This study shows that a high proportion of skin-infiltrating T cells in LSA has a potential cytotoxic function. The results indicate that hydropic degeneration of basal keratinocytes may at least partially be mediated by CTL-dependent mechanisms. Our data also indicate that a cell-mediated immune response may play an important role in the pathogenesis of the disease.