Structure Insights Into Photosystem I Octamer From Cyanobacteria.
Structure Insights Into Photosystem I Octamer From Cyanobacteria.
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蓝藻光系统 I 八聚体的结构洞察
DOI：
10.3389/fmicb.2022.876122
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发表时间：
2022
影响因子：
5.2
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中科院分区：
文献类型：
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光系统低聚物的多样性对于理解光合生物如何适应光照条件至关重要。由于其结构和生理意义，PSI 超级复合物的组装最近在叶绿体和蓝细菌方面引起了极大的兴趣。在本研究中，首次从丝状蓝藻鱼腥藻弱光培养物中分离出两种新型光系统I超复合物。 PCC 7120。通过生物化学和生物物理表征，这些复合物被定义为 PSI 六聚体和八聚体。他们的 77K 发射光谱表明，红色形式的叶绿素在低聚过程中似乎没有受到影响。通过冷冻电镜单粒子分析，解析了 PSI 八聚体的近原子 (7.0 Å) 分辨率结构，并揭示了稳定 PSI 八聚体的分子组装体。
The diversity of photosystem oligomers is essential to understanding how photosynthetic organisms adapt to light conditions. Due to its structural and physiological significance, the assembly of the PSI supercomplex has been of great interest recently in terms of both chloroplast and cyanobacteria. In this study, two novel photosystem I supercomplexes were isolated for the first time from the low light incubated culture of filamentous cyanobacterium Anabaena sp. PCC 7120. These complexes were defined as PSI hexamers and octamers through biochemical and biophysical characterization. Their 77K emission spectra indicated that the red forms of chlorophylls seemed not to be affected during oligomerization. By cryo-EM single-particle analysis, a near-atomic (7.0 Å) resolution structure of a PSI octamer was resolved, and the molecular assemblies of a stable PSI octamer were revealed.