Using genomic information for management planning of an endangered perennial, Viola uliginosa
Using genomic information for management planning of an endangered perennial, Viola uliginosa
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DOI：
10.1002/ece3.6093
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发表时间：
2020-02-17
影响因子：
2.6
通讯作者：
Kvist，Laura
中科院分区：
文献类型：
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作者：
Lee，Kyung Min;Ranta，Pertti;Kvist，Laura
栖息地受到频繁的自然和/或人为变化的物种是保护管理的挑战。我们研究了这样一个物种，紫花地丁，一种濒危的多年生湿地物种，通常栖息在河流/湖泊旁边的零星淹没的草地。为了估计基因组多样性、种群结构和历史，我们使用基因组SNP数据和双酶切限制性位点相关DNA测序（ddRAD‐seq）在芬兰取样了5个位点，在爱沙尼亚取样了3个位点，在斯洛文尼亚、白俄罗斯和波兰各取样了1个位点。我们发现了单系种群，高水平的近亲繁殖（平均种群FSNP = 0.407-0.945），低有效种群大小（Ne= 0.8-50.9），过去人口扩张的迹象，以及罕见的长距离扩散。我们的研究结果对实施保护策略具有重要意义，其中应包括建立种子库，迁地栽培和重新引入适当的起源个体，并结合持续的种群监测和栖息地管理。
Species occupying habitats subjected to frequent natural and/or anthropogenic changes are a challenge for conservation management. We studied one such species,Viola uliginosa, an endangered perennial wetland species typically inhabiting sporadically flooded meadows alongside rivers/lakes. In order to estimate genomic diversity, population structure, and history, we sampled five sites in Finland, three in Estonia, and one each in Slovenia, Belarus, and Poland using genomic SNP data with double‐digest restriction site‐associated DNA sequencing (ddRAD‐seq). We found monophyletic populations, high levels of inbreeding (mean populationFSNP= 0.407–0.945), low effective population sizes (Ne= 0.8–50.9), indications of past demographic expansion, and rare long‐distance dispersal. Our results are important in implementing conservation strategies forV. uliginosa, which should include founding of seed banks, ex situ cultivations, and reintroductions with individuals of proper origin, combined with continuous population monitoring and habitat management.