Congenic strains confirm aerobic running capacity quantitative trait loci on rat chromosome 16 and identify possible intermediate phenotypes.
Congenic strains confirm aerobic running capacity quantitative trait loci on rat chromosome 16 and identify possible intermediate phenotypes.
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DOI：
10.1152/physiolgenomics.00027.2006
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发表时间：
2007-03
影响因子：
4.6
通讯作者：
J. A. Ways;Brian M. Smith;J. Barbato;R. Ramdath;Krista M. Pettee;S. Deraedt;D. Allison;L. Koch;S. J. Lee;G. Cicila
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文献类型：
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作者：
J. A. Ways;Brian M. Smith;J. Barbato;R. Ramdath;Krista M. Pettee;S. Deraedt;D. Allison;L. Koch;S. J. Lee;G. Cicila
在此之前，我们鉴定了两种不同于跑步机有氧运动能力（ARC）的近亲大鼠品系，即低表现的哥本哈根大鼠（COP）和高表现的DA大鼠，并利用F(2)（COPxDA）群体鉴定了大鼠16号染色体（RNO16）和大鼠3号染色体近端部分（RNO3）上的ARC数量性状位点（qtl）。通过将DA大鼠的RNO16和RNO3近端部分渗入到COP大鼠的遗传背景中，我们培育了两株同源大鼠来进一步研究这些ARC qtl，命名为COP。DA（chr 16）和COP。分别为DA（chr 3）。警察。DA（chr 16）大鼠的ARC显著高于COP大鼠（696.7 +/- 38.2 m比571.9 +/- 27.5 m, P = 0.03）。警察。与COP大鼠相比，DA（chr 3）大鼠的ARC （643.6 +/- 40.9 m比571.9 +/- 27.5 m）增加，但不显著。警察。与COP大鼠相比，DA（chr 16）大鼠皮下脂肪显著增加，空腹甘油三酯水平显著降低（P < 0.05），表明导致脂肪代谢品类差异的基因也位于RNO16上。虽然qtl的共定位可能是巧合，但这些能量平衡的差异也可能与COP优越的运行性能有关。DA（chr 16）经济大鼠。
We previously identified two inbred rat strains divergent for treadmill aerobic running capacity (ARC), the low-performing Copenhagen (COP) and the high-performing DA rats, and used an F(2)(COPxDA) population to identify ARC quantitative trait loci (QTLs) on rat chromosome 16 (RNO16) and the proximal portion of rat chromosome 3 (RNO3). Two congenic rat strains were bred to further investigate these ARC QTLs by introgressing RNO16 and the proximal portion of RNO3 from DA rats into the genetic background of COP rats and were named COP.DA(chr 16) and COP.DA(chr 3), respectively. COP.DA(chr 16) rats had significantly greater ARC compared with COP rats (696.7 +/- 38.2 m vs. 571.9 +/- 27.5 m, P = 0.03). COP.DA(chr 3) rats had increased, although not significant, ARC compared with COP rats (643.6 +/- 40.9 m vs. 571.9 +/- 27.5 m). COP.DA(chr 16) rats had significantly greater subcutaneous abdominal fat, as well as decreased fasting triglyceride levels, compared with COP rats (P < 0.05), indicating that genes responsible for strain differences in fat metabolism are also located on RNO16. While this colocalization of QTLs may be coincidental, it is also possible that these differences in energy balance may be associated with the superior running performance of COP.DA(chr 16) consomic rats.