Response regulator VemR regulates the transcription of flagellar rod gene flgG by interacting with σ(54) factor RpoN2 in Xanthomonas citri ssp. citri.
Response regulator VemR regulates the transcription of flagellar rod gene flgG by interacting with σ(54) factor RpoN2 in Xanthomonas citri ssp. citri.
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响应调节因子 VemR 通过与柑橘黄单胞菌中的 sigma(54) 因子 RpoN2 相互作用来调节鞭毛杆基因 flgG 的转录。
DOI：
10.1111/mpp.12762
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发表时间：
2019-03
影响因子：
4.9
通讯作者：
Zou H
中科院分区：
文献类型：
--
作者：
Wu W;Zhao Z;Luo X;Fan X;Zhuo T;Hu X;Liu J;Zou H
柑橘黄单胞菌。柑橘是一种极鞭毛细菌，在世界范围内引起柑橘溃疡病。在本研究中，我们发现柑橘X. citri ssp。柑橘反应调节因子VemR在鞭毛来源的细胞运动中起调节作用。vemR基因的缺失导致细胞活力的降低，以及毒力和胞外多糖产生的减少。逆转录-聚合酶链反应（RT - PCR）表明，vemR与rpoN2和fleQ一起转录在一个操纵子中。在vemR突变体中，鞭毛远端杆基因flgG显著下调。由于flgG也依赖于rpoN2，我们推测VemR和rpoN2物理相互作用，这被酵母双杂交和麦芽糖结合蛋白（MBP）下拉实验证实。这表明flgG的转录受到VemR和RpoN2的协同控制。为了证实这一点，我们构建了一个vemR和rpoN2双突变体。在这个突变体中，细胞活力和flgG转录的减少不能通过单独表达vemR或rpoN2来恢复。相比之下，双突变体中vemR和rpoN2的表达恢复了野生型表型。综上所述，我们的数据表明，响应调节因子VemR作为RpoN2同源激活因子，在柑橘中积极调节杆基因flgG的转录。citri。
Xanthomonas citri ssp. citri, a polar flagellated bacterium, causes citrus canker disease worldwide. In this study, we found that the X. citri ssp. citri response regulator VemR plays a regulatory role in flagellum‐derived cell motility. Deletion of the vemR gene resulted in a reduction in cell motility, as well as reductions in virulence and exopolysaccharide production. Reverse transcription‐polymerase chain reaction (RT‐PCR) demonstrated that vemR is transcribed in an operon together with rpoN2 and fleQ. In the vemR mutant, the flagellar distal rod gene flgG was significantly down‐regulated. Because flgG is also rpoN2 dependent, we speculated that VemR and RpoN2 physically interact, which was confirmed by yeast two‐hybrid and maltose‐binding protein (MBP) pull‐down assays. This suggested that the transcription of flgG is synergistically controlled by VemR and RpoN2. To confirm this, we constructed a vemR and rpoN2 double mutant. In this mutant, the reductions in cell motility and flgG transcription were unable to be restored by the expression of either vemR or rpoN2 alone. In contrast, the expression of both vemR and rpoN2 together in the double mutant restored the wild‐type phenotype. Together, our data demonstrate that the response regulator VemR functions as an RpoN2 cognate activator to positively regulate the transcription of the rod gene flgG in X. citri ssp. citri.
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