Expression of an Arabidopsis sodium/proton antiporter gene (AtNHX1) in peanut to improve salt tolerance
Expression of an Arabidopsis sodium/proton antiporter gene (AtNHX1) in peanut to improve salt tolerance
复制标题
拟南芥钠/质子逆向转运蛋白基因（AtNHX1）在花生中的表达以提高耐盐性
DOI：
10.1007/s11816-011-0200-5
复制
发表时间：
2012-01-01
影响因子：
2.4
通讯作者：
Zhang, Hong
中科院分区：
文献类型：
--
作者：
Banjara, Manoj;Zhu, Longfu;Zhang, Hong
盐度是影响全世界农业生产力的主要环境压力。提高作物耐盐性的一种方法是通过拟南芥基因AtNHX 1的高表达，AtNHX 1编码液泡钠/质子反向转运蛋白，将过量的钠离子隔离到大的细胞内液泡中。将细胞质钠螯合到植物细胞的液泡中导致细胞质中低水平的钠，这最小化了钠毒性和对细胞质中重要酶的损伤。与此同时，钠在液泡中的积累恢复了细胞内环境的正确渗透压，这有利于植物根细胞的水分吸收，并提高了高盐土壤下组织的保水性。为提高高盐胁迫下花生的产量和品质，采用农杆菌介导法将AtNHX 1基因导入花生植株中。表达AtNHX 1的花生植物在高达150 mM NaCl的水平下表现出增加的耐盐性。与野生型植物相比，AtNHX 1表达的花生植物遭受的损害较小，产生更多的生物量，含有更多的叶绿素，并在盐条件下保持较高的光合速率。这些数据表明AtNHX 1可以用于提高花生的耐盐性。
Salinity is a major environmental stress that affects agricultural productivity worldwide. One approach to improving salt tolerance in crops is through high expression of the Arabidopsis gene AtNHX1, which encodes a vacuolar sodium/proton antiporter that sequesters excess sodium ion into the large intracellular vacuole. Sequestering cytosolic sodium into the vacuoles of plant cells leads to a low level of sodium in cytosol, which minimizes the sodium toxicity and injury to important enzymes in cytosol. In the meantime, the accumulation of sodium in vacuoles restores the correct osmolarity to the intracellular milieu, which favors water uptake by plant root cells and improves water retention in tissues under soils that are high in salt. To improve the yield and quality of peanut under high salt conditions, AtNHX1 was introduced into peanut plants through Agrobacterium-mediated transformation. The AtNHX1-expressing peanut plants displayed increased tolerance of salt at levels up to 150 mM NaCl. When compared to wild-type plants, AtNHX1-expressing peanut plants suffered less damage, produced more biomass, contained more chlorophyll, and maintained higher photosynthetic rates under salt conditions. These data indicate that AtNHX1 can be used to enhance salt tolerance in peanut.