CELL PRODUCTION AND DNA ACCUMULATION IN THE WHEAT ENDOSPERM, AND THEIR ASSOCIATION WITH GRAIN WEIGHT
CELL PRODUCTION AND DNA ACCUMULATION IN THE WHEAT ENDOSPERM, AND THEIR ASSOCIATION WITH GRAIN WEIGHT
复制标题
DOI：
10.1093/oxfordjournals.aob.a087263
复制
发表时间：
1986-12-01
期刊：
影响因子：
4.2
通讯作者：
GALE, MD
中科院分区：
文献类型：
--
作者：
CHOJECKI, AJS;BAYLISS, MW;GALE, MD
对中国春和穗型小麦胚乳细胞核DNA含量进行了测定。检测到3C、6C、12 C和24 C核，表明发生了某种形式的核内复制和/或核内多倍化。胚乳中的DNA总量持续增加，直到开花后24天。这种DNA的积累既来自新细胞核的产生，也来自现有细胞核DNA含量的增加。胚乳细胞数的估计是由每个胚乳的总DNA含量和每个胚乳核的平均DNA含量的成熟粒重不同的基因型的范围。胚乳DNA含量和细胞数与成熟粒重均呈正相关。然而，并不是所有的粒重的变化可以归因于细胞数量的变化，因为每个胚乳细胞的平均干重的差异。大粒品种，Spica，有一个更大的平均重量每个胚乳细胞比中国春，这种差异后出现的胚乳细胞生产已经停止。
Nuclear DNA content was measured in developing endosperm cells of two wheat [Triticum aestivum] varieties, Chinese Spring and Spica. 3C, 6C, 12C and 24C nuclei were detected, indicating that some form of endoreduplication and/or endopolyploidization was occurring. The total amount of DNA in the endosperm continued to increase until 24 days post anthesis. This accumulation of DNA resulted both from production of new nuclei and also from increases in the DNA content of existing nuclei. Estimates of endosperm cell numbers were made from the total DNA content per endosperm and the mean DNA content per endosperm nucleus for a range of genotypes differing in mature grain weight. Endosperm DNA content and cell number were both positively associated with mature grain weight among the genotypes examined. However, not all of the variation in grain weight could be attributed to variation in cell number because of differences in mean dry weight per endosperm cell. The large-grained variety, Spica, had a greater mean weight per endosperm cell than Chinese Spring and this difference arose after cell production in the endosperm had ceased.