DEOXYNUCLEOTIDE POOLS AND DNA-SYNTHESIS IN RESTING AND PHA-STIMULATED HUMAN-LYMPHOCYTES TREATED WITH MUTAGENS
DEOXYNUCLEOTIDE POOLS AND DNA-SYNTHESIS IN RESTING AND PHA-STIMULATED HUMAN-LYMPHOCYTES TREATED WITH MUTAGENS
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DOI：
10.1016/0014-4827(92)90444-d
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发表时间：
1992-04-01
影响因子：
3.7
通讯作者：
CELOTTI, L
中科院分区：
文献类型：
--
作者：
FERRARO, P;BIANCHI, V;CELOTTI, L
在用紫外线或 MMS 处理的静息和 PHA 刺激的 PBL 中，我们测量了 dTTP 和 dATP 池的大小以及 ATP 含量的变化作为细胞毒性的指标。通过测量静息 PBL 中的 DNA 修复（非计划 DNA 合成）和受刺激 PBL 中 DNA 复制的抑制来检查对 DNA 合成的影响。虽然两种处理都会影响 DNA 合成，但只有 MMS 扰乱 dNTP 库并降低细胞内 ATP 浓度。所有这些影响在循环中比在静息淋巴细胞中更为明显。
In resting and PHA-stimulated PBL treated with uv light or MMS we measured the sizes of the dTTP and dATP pools and the variation of ATP content taken as an indicator of cytotoxicity. The effects on DNA synthesis were examined by measuring DNA repair (unscheduled DNA synthesis) in resting PBL and the inhibition of DNA replication in stimulated PBL. While both treatments affected DNA synthesis, only MMS perturbed dNTP pools and decreased the intracellular concentration of ATP. All the effects were more evident in cycling than in resting lymphocytes.