PURINE NUCLEOTIDE-METABOLISM IN RESIDENT AND ACTIVATED RAT MACROPHAGES INVITRO
PURINE NUCLEOTIDE-METABOLISM IN RESIDENT AND ACTIVATED RAT MACROPHAGES INVITRO
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DOI：
10.1002/eji.1830150618
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发表时间：
1985-01-01
影响因子：
5.4
通讯作者：
COHEN, A
中科院分区：
文献类型：
--
作者：
BARANKIEWICZ, J;COHEN, A
在驻留的腹膜巨噬细胞（M.PHI.]巯基乙酸盐引起的腹膜M.PHI.体外嘌呤碱基（腺嘌呤、次黄嘌呤和鸟嘌呤）的补救在两种M.PHI中都有活性。种群，而嘌呤从头生物合成是低的。嘌呤核苷（肌苷、鸟苷和腺苷）被有效降解为尿酸，只有腺苷被直接回收为核苷酸。在诱发的M.PHI中，嘌呤补救显著增加。与居民M.PHI.相比，而嘌呤降解途径仅略微增强。这些结果清楚地表明嘌呤碱基的补救是M.PHI中嘌呤核苷酸生物合成的主要来源，但核苷酸催化剂是主要途径。
The overall purine metabolism was studied in detail in resident peritoneal macrophages (M.PHI.] and in thioglycolate elicited peritoneal M.PHI. in vitro. The salvage of purine bases (adenine, hypoxanthine and guanine) was active in both M.PHI. populations, whereas purine biosynthesis de novo was low. Purine nucleosides (inosine, guanosine and adenosine) were efficiently degraded to uric acid and only adenosine was directly salvaged into nucleotides. Purine salvage was markedly increased in elicited M.PHI. as compared to resident M.PHI. whereas purine degradation pathways were enhanced only slightly. These results clearly indicate that salvage of purine bases is the main source for purine nucleotide biosynthesis in M.PHI., but nucleotide catabolism is the predominant pathway.