Nonlinear estimation of Monod growth kinetic parameters from a single substrate depletion curve
Nonlinear estimation of Monod growth kinetic parameters from a single substrate depletion curve
复制标题
从单一底物消耗曲线非线性估计莫诺生长动力学参数
DOI：
10.1128/aem.45.5.1453-1458.1983
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发表时间：
1983
影响因子：
4.4
通讯作者：
J. Tiedje
中科院分区：
文献类型：
--
作者：
J. A. Robinson;J. Tiedje
利用非线性最小二乘分析，将S形底物消耗数据与积分的Monod方程进行拟合，得到Monod生长动力学参数。当初始底物浓度在混阶区时，包含已知测量误差的模拟数据集的非线性估计提供了用于创建这些数据的最大单位单位、Ks和Y值的准确估计。对S形底物耗竭数据的非线性回归分析也适用于脱硫弧菌的H2限制分批生长。菌株G11。积分的Monod方程可以更方便地估计生长动力学参数，特别是对于气相基质，但必须认识到，所获得的mumax、Ks和Y的估计可能受到接种物生长速度历史的影响。
Monod growth kinetic parameters were estimated by fitting sigmoidal substrate depletion data to the integrated Monod equation, using nonlinear least-squares analysis. When the initial substrate concentration was in the mixed-order region, nonlinear estimation of simulated data sets containing known measurement errors provided accurate estimates of the mu max, Ks, and Y values used to create these data. Nonlinear regression analysis of sigmoidal substrate depletion data was also evaluated for H2-limited batch growth of Desulfovibrio sp. strain G11. The integrated Monod equation can be more convenient for the estimation of growth kinetic parameters, particularly for gaseous substrates, but it must be recognized that the estimates of mu max, Ks, and Y obtained may be influenced by the growth rate history of the inoculum.