Molecular cloning of a novel secreted peptide, INM02, and regulation of its expression by glucose
Molecular cloning of a novel secreted peptide, INM02, and regulation of its expression by glucose
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新型分泌肽 INM02 的分子克隆及其表达的葡萄糖调节
DOI：
10.1677/joe-09-0086
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发表时间：
2009-09-01
影响因子：
4
通讯作者：
Hu, Renming
中科院分区：
文献类型：
--
作者：
Wang, Xuanchun;Gong, Wei;Hu, Renming
我们报告鉴定一种新的分泌肽，INM02。人INM02基因的mRNA转录物约为3.0 kb。它的开放阅读框包含762个bps，编码254个氨基酸的蛋白质。Northern blot分析表明，INM02 mRNA在大鼠组织中广泛表达，特别是在胰岛、睾丸和膀胱组织中表达量丰富。我们表达了重组INM02蛋白，并获得了兔抗INM02多克隆抗体。我们在此证明INM02可以通过ELISA在人血清中检测到。我们也提出证据表明，INM02 mRNA的表达可能受到葡萄糖的调节。在MIN6细胞和完整的离体胰岛上进行的实验表明，高糖（25 mM）使INM02 mRNA水平比低糖（5-5 mM）增加了三倍以上。ELISA分析显示，体外高糖可显著增强INM02的分泌。推测INM02作为一种新型分泌蛋白，与胰岛特别是β细胞的功能有关。内分泌学杂志（2009）202,355-364
We report the identification of a novel secreted peptide, INM02. The mRNA transcript of human INM02 gene is about 3.0 kb. Its open-reading frame contains 762 bps and encodes a protein of 254 amino acids. Northern blot analysis demonstrates that INM02 mRNA is widely expressed in rat tissues, especially with abundant quantities in pancreatic islets, testis, and bladder tissue. We have expressed recombinant INM02 protein and generated rabbit anti-INM02 polyclonal antibodies. We show here that INM02 could be detectable in human serum by ELISA. We also present evidence that INM02 mRNA expression could be regulated by glucose. Experiments on both MIN6 cells and intact isolated islets demonstrate that INM02 mRNA levels are increased more than threefold by high glucose (25 mM) when compared with low glucose (5-5 mM). ELISA analysis shows that secretion of INM02 is significantly augmented by high glucose in vitro. It is speculated that as a novel secreted protein, INM02 is associated with functions of pancreatic islets, especially of beta-cells. Journal of Endocrinology (2009) 202, 355-364