Survival of contagious ovine digital dermatitis (CODD)-associated treponemes on disposable gloves after handling CODD-affected feet.
Survival of contagious ovine digital dermatitis (CODD)-associated treponemes on disposable gloves after handling CODD-affected feet.
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DOI：
10.1136/vr.104228
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发表时间：
2017-07-22
期刊：
影响因子：
--
通讯作者：
Evans NJ
中科院分区：
文献类型：
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作者：
Angell JW;Clegg SR;Grove-White DH;Blowey RW;Carter SD;Duncan JS;Evans NJ
羊传染性趾皮炎（CODD）和牛传染性趾皮炎（BDD）分别是绵羊和牛传染性跛行的原因，并且与特定密螺旋体的存在密切相关，通常分离出三种不同的可培养密螺旋体群：密螺旋体培养基、噬菌体密螺旋体和足密螺旋体。本研究的目的是研究在处理明显临床受累动物时通过手套传播CODD相关密螺旋体的可能性。作为常规检查的一部分，用戴手套的手处理有和没有CODD的羊的脚。然后在处理后立即擦拭手套以检测密螺旋体的存在。检测方法包括培养和分离技术以及PCR检测DNA。此外，还测定了在空气中的存活时间以及常用消毒剂去除手套上密螺旋体的效力。在这项研究中，我们证明了CODD相关的密螺旋体可以在用于处理受CODD影响的绵羊的脚的手套上存活，但可以使用普通消毒剂有效地去除。这些数据提供了潜在传播途径的证据，并确定了降低这种风险的实用方法。
Both contagious ovine digital dermatitis (CODD) and bovine digital dermatitis (BDD) are causes of infectious lameness in sheep and cattle, respectively, and are strongly associated with the presence of specific treponemes, with three different cultivable phylogroups commonly isolated:Treponema medium,Treponema phagedenisandTreponema pedis. The aim of this study was to investigate the potential to transmit CODD‐associatedTreponemaspecies via gloves used when handling visibly clinically affected animals. The feet of sheep with and without CODD were handled as part of routine examination with gloved hands. The gloves were then swabbed to detect the presence of treponemes immediately after handling. Detection methods included culture and isolation techniques together with DNA detection by PCR. In addition, the duration of survival in air was determined as well as the efficacy of common disinfectants to remove treponemes from gloves. In this study, we demonstrate that CODD‐associated treponemes can survive on gloves used to handle the feet of CODD‐affected sheep but may be removed effectively using common disinfectants. These data provide evidence of a potential route of transmission and identify a practical method to reduce this risk.