Developmental expression of NADPH phagocytic oxidase components in mouse embryos
Developmental expression of NADPH phagocytic oxidase components in mouse embryos
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DOI：
10.1203/00006450-199908000-00004
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发表时间：
1999-08-01
影响因子：
3.6
通讯作者：
Bringas, P
中科院分区：
文献类型：
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作者：
Baehner, RL;Millar-Groff, S;Bringas, P
我们希望确定四种NADPH氧化酶成分p22吞噬氧化酶（phox）、gp91 phox、p47 phox、p67 phox中的每一种何时首次在胚胎学上表达，以及表达模式是否以一致的时间序列发生，或者这四种基因是否同时表达。其中任何一种的缺乏都会导致慢性肉芽肿病（CGD）。在小鼠胚胎植入时（E5.5）至E11.5，以及从E14至足月（E19）在胎肝、脾和肢骨髓中监测p22 phox、gp91 phox、p47 phox、p67 phox的mRNA转录物和蛋白表达。我们观察到，在卵黄囊造血开始之前（E 8.0），p22 phox在E 5.5、p67 phox在E 7.0和p47 phox在E 7.5首先表达mRNA。gp91 Phox mRNA首先在E 9.0表达。然而，只有p22 phox蛋白表达在循环血细胞由E 9.0。没有其他胚胎组织含有phox蛋白之前或之后的血细胞循环的建立。4种特异性mRNA转录物和phox蛋白在E14时在肝脏中发育中的粒细胞巢中表达，并且在E16和E19时在肝脏中继续表达。脾脏和四肢骨髓结果不一致。脐带血中性粒细胞含有所有phox蛋白：这些研究证实，NADPH氧化酶的四种CGD相关phox mRNA组分在胚胎发育早期表达，并且表达以一致的顺序方式发生，但只有p22 phox蛋白出现在胚胎成血细胞中。
We wished to determine when each of the four NADPH oxidase components p22 phagocytic oxidase (phox), gp91 phox, p47 phox, p67 phox is first expressed embryologically and whether the expression pattern occurs in a consistent temporal sequence or whether the four genes are expressed simultaneously. A deficiency of any one of them results in chronic granulomatous disease (CGD). mRNA transcripts and protein expression for p22 phox, gp91 phox, p47 phox, p67 phox was monitored in murine embryos at time of implantation (E5.5) until E 11.5, and in fetal liver, spleen, and limb bone marrow from E 14 until term (E 19). We observed that mRNA was first expressed for p22 phox at E 5.5, for p67 phox at E 7.0 and for p47 phox at E 7.5 before the onset of yolk sac hematopoiesis (E 8.0). gp91 Phox mRNA was first expressed at E 9.0. However, only p22 phox protein was expressed in circulating hemocytoblast by E 9.0. No other embryonic tissue contained phox proteins either before or after the establishment of hemocytoblastic circulation. The four specific mRNA transcripts and phox proteins were expressed in nests of developing granulocytes in liver by E 14 and the expression continued in the liver at E 16 and E 19. Spleen and limb bone marrow showed inconsistent results. Cord blood neutrophils contained all phox proteins: These studies confirm that the four CGD-related phox mRNA components of NADPH oxidase are expressed early in embryonic development and the expression occurs in a consistent sequential fashion but only p22 phox protein appears in embryonic hemocytoblast.