Cytoplasmic Streaming Velocity as a Plant Size Determinant
Cytoplasmic Streaming Velocity as a Plant Size Determinant
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DOI：
10.1016/j.devcel.2013.10.005
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发表时间：
2013-11-11
影响因子：
11.8
通讯作者：
Ito, Kohji
中科院分区：
文献类型：
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作者：
Tominaga, Motoki;Kimura, Atsushi;Ito, Kohji
细胞质流动是一种主动运输，广泛存在于从藻类到被子植物的各种植物细胞中。尽管已发现细胞器相关的肌球蛋白XI沿肌动蛋白束运动产生细胞质流动，但其在植物中的基本功能仍不清楚。我们通过用珊瑚轮藻肌球蛋白XI和智人肌球蛋白VB分别取代拟南芥肌球蛋白XI-2的运动域，产生了高速和低速嵌合肌球蛋白XI。令人惊讶的是，表达高速和低速嵌合肌球蛋白XI-2的转基因拟南芥的植株大小分别比野生型植株大和小。这种大小的变化分别与细胞质流动的加速和减速有关。我们的结果强烈地表明，细胞质流动是植物大小的关键决定因素。此外，由于细胞质流动是植物细胞内运输的常见系统，我们的系统可以应用于植物的人工大小控制。
Cytoplasmic streaming is active transport widely occurring in plant cells ranging from algae to angiosperms. Although it has been revealed that cytoplasmic streaming is generated by organelle-associated myosin XI moving along actin bundles, the fundamental function in plants remains unclear. We generated high- and low-speed chimeric myosin XI by replacing the motor domains of Arabidopsis thaliana myosin XI-2 with those of Chara corallina myosin XI and Homo sapiens myosin Vb, respectively. Surprisingly, the plant sizes of the transgenic Arabidopsis expressing high- and low-speed chimeric myosin XI-2 were larger and smaller, respectively, than that of the wild-type plant. This size change correlated with acceleration and deceleration, respectively, of cytoplasmic streaming. Our results strongly suggest that cytoplasmic streaming is a key determinant of plant size. Furthermore, because cytoplasmic streaming is a common system for intracellular transport in plants, our system could have applications in artificial size control in plants.