Biosynthesis of Cr(III) nanoparticles from electroplating wastewater using chromium-resistant Bacillus subtilis and its cytotoxicity and antibacterial activity

Biosynthesis of Cr(III) nanoparticles from electroplating wastewater using chromium-resistant Bacillus subtilis and its cytotoxicity and antibacterial activity
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DOI:
10.1080/21691401.2016.1228660
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发表时间:
2017-01-01
影响因子:
5.8
通讯作者:
Kamala-Kannan, S.
Kamala-Kannan, S.
中科院分区:
工程技术2区
文献类型:
--
作者:
Kanakalakshmi, A.;Janaki, V.;Kamala-Kannan, S.

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本研究的目的是合成和表征铬(III)纳米粒子从电镀工业废水和耐铬枯草芽孢杆菌。Cr(III)纳米颗粒的形成通过在300 nm处的紫外-可见(UV-Vis)光谱来证实。纳米颗粒的尺寸从4到50 nm变化,能量色散谱图显示出强的Cr峰,约在4.45和5.2keV。纳米颗粒抑制致病菌金黄色葡萄球菌和大肠杆菌的生长。使用HEK 293细胞研究了合成的Cr(III)纳米颗粒的细胞毒性作用,并且发现细胞活力随着Cr(III)纳米颗粒浓度的增加而降低。
The aim of this study was to synthesize and characterize Cr(III) nanoparticles using wastewater from electroplating industries and chromium-resistant Bacillus subtilis. Formation of Cr(III) nanoparticles was confirmed by UV-visible (UV-Vis) spectroscopy at 300nm. The size of the nanoparticles varied from 4 to 50nm and energy dispersive spectroscopy profile shows strong Cr peak approximately at 4.45 and 5.2keV. The nanoparticles inhibited the growth of pathogenic bacteria Staphylococcus aureus and Escherichia coli. The cytotoxic effect of the synthesized Cr(III) nanoparticle was studied using HEK 293 cells, and the cell viability was found to decrease with increasing concentration of Cr(III) nanoparticles.