Discrimination of Zhishi from different species using rapid-resolution liquid chromatography-diode array detection/ultraviolet (RRLC-DAD/UV) coupled with multivariate statistical analysis

Discrimination of Zhishi from different species using rapid-resolution liquid chromatography-diode array detection/ultraviolet (RRLC-DAD/UV) coupled with multivariate statistical analysis
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使用快速分辨率液相色谱-二极管阵列检测/紫外线 (RRLC-DAD/UV) 结合多变量统计分析区分不同物种的枳实

DOI:
10.5897/jmpr11.1504
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发表时间:
2012-02
影响因子:
--
通讯作者:
刘元艳
刘元艳
中科院分区:
--
文献类型:
--
作者:
刘元艳

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枳实是最常用的传统中药之一。许多柑桔属植物,如枳壳及其栽培品种、甜橙及其栽培品种、野橙等,都可用作枳实。但不同种属的枳实在疗效上存在差异。此外,由于它们的形态相似,很难区分物种。 采用快速分离液相色谱-二极管阵列检测器/紫外(RRLC-DAD/UV)法测定了77个3种柑橘类果实样品中4种黄酮类化合物(柚皮苷、柚皮苷、橙皮苷和新橙皮苷)的含量。主成分分析(PCA)的定量数据表明,明确的分离组成之间的三个品种的芝石样品。此外,所建立的差分模式随后被应用于识别未知的商业样品的来源。   关键词:枳实,柑桔属植物,黄酮,快速分离液相色谱,多元统计分析。
Zhishi is one of the most commonly used traditional Chinese medicinal plants. Many species ofCitrus plants, such as Citrus aurantium L. and its cultivars, Citrus sinensis Osbeck and its cultivars, and Citrus junos Sieb. ex Tanaka, can be used as Zhishi. However, Zhishi from different species exhibited differences in their therapeutic effects. In addition, it was very difficult to distinguish the species due to their similar morphologies. The contents of four flavanones (narirutin, naringin, hesperidin and neohesperidin) of 77 Zhishi samples from threeCitrus species were determined using rapid resolution liquid chromatography-diode array detection/UV (RRLC-DAD/UV) and multivariate statistical analysis. Principal component analysis (PCA) of the quantitative data showed a clear separation of compositions among the three species of Zhishi samples. Furthermore, the established differential mode was subsequently applied to identify the origin of unknown commercial samples.   Key words: Zhishi, Citrus species, flavanone, rapid resolution liquid chromatography (RRLC), multivariate statistical analysis.
DOI: 10.1248/yakushi1947.108.10_1008
发表时间: 1988-10
期刊: Yakugaku zasshi : Journal of the Pharmaceutical Society of Japan
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K. Hosoda;M. Noguchi
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DOI: --
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影响因子: --
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