Novel in situ visualisation of rat intestinal absorption of polyphenols via matrix-assisted laser desorption/ionisation mass spectrometry imaging
Novel in situ visualisation of rat intestinal absorption of polyphenols via matrix-assisted laser desorption/ionisation mass spectrometry imaging
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DOI：
10.1038/s41598-019-39405-w
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发表时间：
2019-02-28
影响因子：
4.6
通讯作者：
Matsui, Toshiro
中科院分区：
文献类型：
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作者：
Huu-Nghi Nguyen;Tanaka, Mitsuru;Matsui, Toshiro
基质辅助激光解吸/电离质谱成像（MALDI-MSI）目前用于器官靶点可视化的生理学评价。在本研究中，MALDI-MSI被用作可视化技术来研究多酚的肠吸收。进行硝苯地平/植酸辅助的MALDI-MSI以在大鼠空肠中可视化茶黄素-3'-O-没食子酸酯（TF3'G）和表儿茶素-3-O-没食子酸酯（ECG），进行50 μ M、60分钟的转运实验。在顶端区域成功观察到不可吸收的TF 3'G，而在整个大鼠空肠中检测到可吸收的ECG。还进行了MALDI-MSI以确定靶代谢物的转运途径。用靶向单羧酸转运蛋白和有机阴离子转运多肽的抑制剂处理后，膜中对应于TF3 ′ G和ECG的信号减弱。通过抑制外排途径实现了TF3'G的增强可视化。我们的研究结果表明，目前的MALDI-MSI可以提供关键的空间信息，肠吸收的目标，TF3 'G和ECG被纳入肠组织，然后流出回到顶端室。此外，MALDI-MSI分析表明，TF 3 ′ G在流入/流出过程中对II相代谢具有抗性，而ECG对甲基化和硫酸化反应敏感。总之，辅助MALDI-MSI可以作为一个强大的原位可视化技术验证肠道运输途径和调查代谢的促排剂。
Matrix-assisted laser desorption/ionisation mass spectrometry imaging (MALDI-MSI) is presently used in physiological evaluations for visualisation of targets in organs. In the present study, MALDI-MSI was used as a visualisation technique to investigate the intestinal absorption of polyphenols. Nifedipine/phytic acid-aided MALDI-MSI was performed to visualise theaflavin-3'-O-gallate (TF3'G) and epicatechin-3-O-gallate (ECG) in the rat jejunum for 50-mu M, 60-min transport experiments. Non-absorbable TF3'G was successfully visualised at the apical region, whereas absorbable ECG was detected throughout the rat jejunum. MALDI-MSI was also performed to determine the transport routes of the target metabolites. Signals corresponding to TF3'G and ECG in the membranes were diminished following treatment with inhibitors targeting the monocarboxylic acid transporter and organic anion transporting polypeptides. Enhanced visualisation of TF3'G was achieved by inhibiting efflux routes. Our findings demonstrated that the present MALDI-MSI can provide critical spatial informations on intestinal absorption of targets, by which TF3'G and ECG were incorporated into intestinal tissues, followed by efflux back to the apical compartment. In addition, MALDI-MSI analyses suggested that TF3'G was resistant to phase II metabolism during the influx/efflux processes, whereas ECG was susceptible to methylation and sulphation reactions. In conclusion, inhibitor-aided MALDI-MSI could serve as a powerful in situ visualisation technique for verifying intestinal transport routes and investigating the metabolism of penetrants.