Multiple mechanisms of CB1 cannabinoid receptors regulation
Multiple mechanisms of CB1 cannabinoid receptors regulation
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DOI：
10.1016/s0006-8993(03)02970-6
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发表时间：
2003-08-08
期刊：
影响因子：
2.9
通讯作者：
Sarne, Y
中科院分区：
文献类型：
--
作者：
Keren, O;Sarne, Y
在转CB1受体的HEK-293细胞和自然表达CB1受体的神经母细胞瘤N18TG2细胞中，检测了激动剂对大麻素CB1受体的诱导调节。在HEK-293细胞中，CB1受体的内化通过笼蛋白包裹的小凹和小窝平行进行。两条通路同时中断可诱导代偿内吞机制(S)。在N18TG2细胞中，内吞作用不是由小凹样膜结构域介导的。异源阿片类药物诱导的CB1受体下调在HEK-293细胞中很明显，但在N18TG2细胞中不明显。这些数据表明，CB1受体的调节存在多条途径。(C)2003爱思唯尔B.V.保留所有权利。
Agonist-induced regulation of cannabinoid CB1 receptors was examined in HEK-293 cells transfected with CB1 receptors and in neuroblastoma N18TG2 cells that naturally express CB1 receptors. In HEK-293 cells, CB1 receptors internalization proceeded, in parallel, via clathrin-coated pits and caveolae. Simultaneous disruption of both pathways induced compensatory endocytic mechanism(s). In N18TG2 cells, endocytosis was not mediated by caveolae-like membrane domains. Heterologous, opioid-induced, downregulation of CB1 receptors was evident in HEK-293 but not N18TG2 cells. The data demonstrate the existence of multiple pathways of CB1 receptors regulation. (C) 2003 Elsevier B.V. All rights reserved.