Nutrient-Dependent Trade-Offs between Ribosomes and Division Protein Synthesis Control Bacterial Cell Size and Growth.
Nutrient-Dependent Trade-Offs between Ribosomes and Division Protein Synthesis Control Bacterial Cell Size and Growth.
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DOI：
10.1016/j.celrep.2020.108183
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发表时间：
2020-09
期刊：
影响因子：
8.8
通讯作者：
D. Serbanescu;Nikola Ojkic;Shiladitya Banerjee
中科院分区：
文献类型：
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作者：
D. Serbanescu;Nikola Ojkic;Shiladitya Banerjee
细胞大小的控制来自于细胞生长和分裂速率之间的调节平衡。在细菌中，简单的定量规律将细胞生长速率与核糖体丰度联系起来。然而，人们对翻译如何在生长扰动下调节细菌细胞的大小和形状仍然知之甚少。在这里，我们开发了棒状细菌生长动力学的全细胞模型，将核糖体丰度与细胞几何形状、分裂控制和细胞外环境联系起来。我们的研究表明，在营养扰动下细胞大小的维持需要核糖体和分裂蛋白合成之间的平衡权衡。在翻译抑制下预测这种权衡关系的偏差，导致不同模式的细胞形态变化，与单细胞实验数据一致。此外，通过用实验数据校准我们的模型，我们预测如何选择营养、翻译和形状扰动的组合来优化细菌的生长适应性和抗生素耐药性。
Cell size control emerges from a regulated balance between the rates of cell growth and division. In bacteria, simple quantitative laws connect cellular growth rate to ribosome abundance. However, it remains poorly understood how translation regulates bacterial cell size and shape under growth perturbations. Here, we develop a whole-cell model for growth dynamics of rod-shaped bacteria that links ribosomal abundance with cell geometry, division control, and the extracellular environment. Our study reveals that cell size maintenance under nutrient perturbations requires a balanced trade-off between ribosomes and division protein synthesis. Deviations from this trade-off relationship are predicted under translation inhibition, leading to distinct modes of cell morphological changes, in agreement with single-cell experimental data onEscherichia coli. Furthermore, by calibrating our model with experimental data, we predict how combinations of nutrient-, translational-, and shape perturbations can be chosen to optimize bacterial growth fitness and antibiotic resistance.