Wood formation in Norway spruce studied by pinning technique and intact tissue sampling method
Wood formation in Norway spruce studied by pinning technique and intact tissue sampling method
复制标题
DOI：
--
复制
发表时间：
2007
期刊：
影响因子：
1.3
通讯作者：
J. Gričar;M. Zupančič;K. Čufar;P. Oven
中科院分区：
文献类型：
--
作者：
J. Gričar;M. Zupančič;K. Čufar;P. Oven
在 2003 年生长季期间，对来自低地森林 Sorsko polje（海拔 350 m）和斯洛文尼亚亚高山地点 Pokljuka（海拔 1200 m）的挪威云杉（云杉）的木质部形成的季节动态进行了细胞水平的研究。每周一次进行钉扎技术和完整组织取样，以评估两种方法检查的有效性。木材形成。钉扎诱导不同分化木质部的干燥、形成层细胞和未分化木质部衍生物的坏死以及愈伤组织、创伤性树脂管和伤口木材的形成。位于愈伤组织内的形成层细胞和径向扩张细胞之间的边界是定义从受伤时达到的木质部增量的可靠标记。完整的组织取样对于精确研究形成层活动、管胞的各个发育阶段以及次生韧皮部的形成具有优越性。
Seasonal dynamics of xylem formation were studied at a cellular level in Norway spruce (Picea abies) from the lowland forests Sorsko polje (350 m a.s.l.) and the sub-alpine site Pokljuka (1200 m a.s.l.) in Slovenia during the growing season 2003. Th e pinning technique and sampling of intact tissue were performed at weekly intervals to assess the usefulness of both methods for examining wood formation. Pinning induced desiccation of diff differentiated xylem, necrosis of cambial cells and undifferentiated xylem derivatives, and formation of callus, traumatic resin canals and wound-wood. The border between cambial cells and radially expanding cells located inside the callus was a reliable mark for defining the xylem increment reached from the time of wounding. Intact tissue sampling was superior for precise investigations of cambial activity, various developmental stages of tracheids,and for secondary phloem formation.