Genetic mapping of the three-pistil gene Pis1 in an F2 population derived from a synthetic hexaploid wheat using multiple molecular marker systems
Genetic mapping of the three-pistil gene Pis1 in an F2 population derived from a synthetic hexaploid wheat using multiple molecular marker systems
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DOI：
10.1007/s42976-020-00078-1
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发表时间：
2020-09
影响因子：
1.6
通讯作者：
Z. Yu;Q. Luo;Z. Peng;S. Wei;Z. Yang;Naoki Yamamoto
中科院分区：
文献类型：
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作者：
Z. Yu;Q. Luo;Z. Peng;S. Wei;Z. Yang;Naoki Yamamoto
The wheat spike mutant 'three-pistil' (TP) exhibits normal external morphology but produces two additional pistils per floret. Much attention has been paid to this natural mutation because elucidation of its genetic foundation potentially paves a way to increase the number of grains per spike for breeding. Our previous studies roughly mapped the causal locus of TP on chromosome 2D. In this study, we performed genetic mapping of the three-pistil genePis1in an F2population derived from a cross of TP and synthetic hexaploid wheat of which chromosome 2D was fromAegilops tauschii. Segregation analysis in the F2population confirmed the dominance of thePis1locus. Bulked segregant analysis with three DNA marker systems revealed a total of 18 markers genetically linked withPis1. Successive genetic linkage analysis of the F2individuals using the 18 markers identified two sequence-related amplified polymorphisms markers,Me5-eM33andMe26-eM33, flanked thePis1locus at distances of 0.6 and 1.7 cM, respectively. Integration of the genetic linkage and previously discovered DNA markers associated withPis1revealed a potential location of thePis1locus betweenMe5-eM33and a KASP markerM75. Thus, the constructed partial genetic linkage map is useful for the isolation ofPis1and marker-assisted breeding in wheat.
