Toxicity of peptides of Amanita virosa mushrooms in mice.
Toxicity of peptides of Amanita virosa mushrooms in mice.
复制标题
毒伞菌肽对小鼠的毒性。
DOI：
10.1016/0272-0590(85)90151-4
复制
发表时间：
1985
期刊：
影响因子：
--
通讯作者：
M. Côté
中科院分区：
文献类型：
--
作者：
A. Loranger;B. Tuchweber;C. Gicquaud;S. St.;M. Côté
本文研究了毒蘑菇鹅膏菌（Amanitavirosamushrooms）中分离的环肽类病毒毒素（ala-viroidin、viroisin、deoxoviroisin、viroidin、deoxoviroidin）和鬼笔环肽（phalloidin）急性中毒小鼠的肝脏反应。将纯化的组分以不同剂量腹腔内给药，以测定LD 50，其范围为1.0至5.3 mg/kg，其中病毒素、鬼笔环肽和病毒素最有效。病毒毒素和鬼笔环肽引起肝脏出血性坏死。肝脏病变的发展是伴随着血清丙氨酸氨基转移酶（ALT）活性的增强以及光镜和电镜的变化。在另外的组中，将胆管插管，并在注射肽（lmg/kg）后收集胆汁2小时以确定其胆汁淤积潜力。肝细胞最早的变化是质膜内陷和胞浆空泡形成。在后期，红细胞聚集在空泡和细胞质中明显。肝损害的严重程度，通过形态学分析判断，与血清ALT活性相关。测试的两种肽（病毒素和鬼笔环肽）使胆汁流量比对照值减少50%以上。在胆汁淤积的发展过程中，观察到肝细胞胆小管极的轻度超微结构改变。由于病毒毒素，如鬼笔环肽，结合到肌动蛋白，这是可能的，他们的亲和力细胞肌动蛋白可能是负责他们的肝毒性。
A study was performed on the hepatic reaction of mice to acute intoxication with virotoxins (ala-viroidin, viroisin, deoxoviroisin, viroidin, deoxoviroidin) and phalloidin, cyclic peptides isolated fromAmanita virosamushrooms. Purified fractions were administered intraperitoneally at various dosages to determine the LD50which ranged from 1.0 to 5.3 mg/kg, with viroidin, phalloidin, and viroisin being the most potent. The virotoxins and phalloidin induced hemorrhagic necrosis of the liver. The development of hepatic lesions was followed by enhanced serum alanine ami-notransferase (ALT) activity as well as by light and electron microscopic changes. In additional groups, bile ducts were cannulated and bile was collected for 2 hr after injection of the peptides (1 mg/kg) to determine their cholestatic potential. The earliest changes in hepatocytes were plasma membrane invagination and cytoplasmic vacuole formation. At later time periods, erythrocyte accumulation was evident in vacuoles and in the cytoplasm. The severity of hepatic damage, as judged by morphologic analysis, was correlated with serum ALT activity. Two of the peptides tested (viroisin and phalloidin) decreased bile flow by more than 50% over control values. Mild ultrastructural alterations in the bile canalicular pole of hepatocytes were observed during the development of cholestasis. Since virotoxins, like phalloidin, are bound to actin, it is possible that their affinity to cellular actin may be responsible for their hepatotoxicity.
在细胞外钙存在和不存在的情况下鬼笔环肽中毒肝细胞的超微结构病理学。
DOI：
--
发表时间：
1982
期刊：
The American journal of pathology
影响因子：
--
作者：
Russo,MA;Kane,AB;Farber,JL
通讯作者：
Farber,JL