Sequential recruitment and combinatorial assembling of multiprotein complexes in transcriptional activation
Sequential recruitment and combinatorial assembling of multiprotein complexes in transcriptional activation
复制标题
DOI：
10.1103/physrevlett.96.198102
复制
发表时间：
2006-05-19
影响因子：
8.6
通讯作者：
Glass, Leon
中科院分区：
文献类型：
--
作者：
Lemaire, Vincent;Lee, Chiu Fan;Glass, Leon
在人类细胞中，雌激素信号诱导特定蛋白质与DNA的周期性结合和解离，以激活雌激素应答基因的转录。这些振荡可以通过假设大量的连续反应来建模，这些连续反应由具有随机动力学速率的线性动力学表示。应用该模型的实验数据预测强大的结合序列，其中蛋白质与DNA在几个不同的振荡阶段。我们的方法规避需要得到详细的动力学图，并适用于其他振荡的生物过程，涉及大量的连续步骤。
In human cells, estrogenic signals induce cyclical association and dissociation of specific proteins with the DNA in order to activate transcription of estrogen-responsive genes. These oscillations can be modeled by assuming a large number of sequential reactions represented by linear kinetics with random kinetic rates. Application of the model to experimental data predicts robust binding sequences in which proteins associate with the DNA at several different phases of the oscillation. Our methods circumvent the need to derive detailed kinetic graphs, and are applicable to other oscillatory biological processes involving a large number of sequential steps.