INITIATION OF HUMAN PARTURITION .12. BIOSYNTHESIS AND METABOLISM OF PROSTAGLANDINS IN HUMAN-FETAL MEMBRANES AND UTERINE DECIDUA
INITIATION OF HUMAN PARTURITION .12. BIOSYNTHESIS AND METABOLISM OF PROSTAGLANDINS IN HUMAN-FETAL MEMBRANES AND UTERINE DECIDUA
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DOI：
10.1016/0002-9378(81)90312-4
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发表时间：
1981-01-01
影响因子：
9.8
通讯作者：
JOHNSTON, JM
中科院分区：
文献类型：
--
作者：
OKAZAKI, T;CASEY, ML;JOHNSTON, JM
以[14C]花生四烯酸为底物，测定了人胎膜和子宫蜕膜组织匀浆中前列腺素合成酶的活性。自然分娩和阴道分娩后的羊膜、绒毛膜和真皮蜕膜组织中PG合成酶的平均比活力分别为1.47、0.53和0.39nmol.倍。在这些临产前择期剖宫产的组织中，PG合成酶的平均比活性分别为0.61、0.33和0.28。产后采集的羊膜PG合成酶比活力显著高于产前(P<；0.02)，羊膜PG合成酶活性高于产前(P<0.05)。乙酰水杨酸和消炎痛对羊膜、绒毛膜和真皮蜕膜PG的合成有抑制作用。PGE_2在羊膜和绒毛膜中形成，而PGE_2和PGF_2·α在羊膜和绒毛膜中形成。是在真性蜕膜中形成的。对NAD+依赖的15-羟基前列腺素脱氢酶进行了活性测定，分娩前后绒毛和蜕膜组织中该酶的比活性较高，羊膜中的15-羟基前列腺素脱氢酶活性较低。
Prostaglandin [PG] synthetase activity was assayed in homogenates prepared from human fetal membranes and uterine decidua tissues with [14C]arachidonic acid used as substrate. In amnion, chorion laeve and decidua vera tissues obtained after spontaneous onset of labor and vaginal delivery the mean specific activities of PG synthetase were respectively 1.47, 0.53 and 0.39 nmol .times. h per g tissue; in these same tissues obtained at elective cesarean section before the onset of labor the mean specific activities of PG synthetase were respectively 0.61, 0.33 and 0.28. The specific activity of PG synthetase of amnion (but not chorion laeve or decidua vera) was significantly greater in samples obtained after labor than in those obtained before labor (P < 0.02). PG synthesis in amnion, chorion laeve and decidua vera was inhibited by acetylsalicylic acid and by indomethacin. PGE2 was formed in amnion and chorion leave but PGE2 and PGF2.alpha. were formed in decidua vera. The activity of NAD+-dependent 15-hydroxyprostaglandin dehydrogenase was assayed and the specific activities of this enzyme in chorion laeve and decidua vera tissues obtained before and after labor were high; 15-hydroxyprostaglandin dehydrogenase activity in amnion was low.