Monoterpenes in the glandular trichomes of tomato are synthesized from a neryl diphosphate precursor rather than geranyl diphosphate
Monoterpenes in the glandular trichomes of tomato are synthesized from a neryl diphosphate precursor rather than geranyl diphosphate
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DOI：
10.1073/pnas.0904113106
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发表时间：
2009-06-30
影响因子：
11.1
通讯作者：
Pichersky, Eran
中科院分区：
文献类型：
--
作者：
Schilmiller, Anthony L.;Schauvinhold, Ines;Pichersky, Eran
我们鉴定了一种顺式异戊二烯基转移酶基因，橙花基二磷酸合酶 1 (NDPS1)，该基因在栽培番茄 (Solanum lycopersicum) 品种 M82 VI 型腺毛中表达，并编码一种催化异戊烯基二磷酸和二甲基烯丙基二磷酸形成橙花基二磷酸的酶。在这些腺体中还鉴定出了萜烯合酶基因水芹烯合酶 1 (PHS1) 的 mRNA。它编码一种酶，使用橙花基二磷酸产生 β-水芹烯作为主要产物以及各种其他单萜。 PHS1 产生的单萜的特征与 VI 型腺体中发现的单萜相同。 PHS1 和 NDPS1 映射到 8 号染色体，并且源自茄属 LA0716 的 8 号染色体片段的存在导致 M82 腺体单萜模式转变为 LA0716 植物的特征。数据表明，与教科书上将香叶基二磷酸作为单萜合酶的“通用”底物的观点相反，在番茄腺中，橙花基二磷酸充当单萜合成的前体。
We identified a cis-prenyltransferase gene, neryl diphosphate synthase 1 (NDPS1), that is expressed in cultivated tomato ( Solanum lycopersicum) cultivar M82 type VI glandular trichomes and encodes an enzyme that catalyzes the formation of neryl diphosphate from isopentenyl diphosphate and dimethylallyl diphosphate. mRNA for a terpene synthase gene, phellandrene synthase 1 (PHS1), was also identified in these glands. It encodes an enzyme that uses neryl diphosphate to produce beta-phellandrene as the major product as well as a variety of other monoterpenes. The profile of monoterpenes produced by PHS1 is identical with the monoterpenes found in type VI glands. PHS1 and NDPS1 map to chromosome 8, and the presence of a segment of chromosome 8 derived from Solanum pennellii LA0716 causes conversion from the M82 gland monoterpene pattern to that characteristic of LA0716 plants. The data indicate that, contrary to the textbook view of geranyl diphosphate as the "universal'' substrate of monoterpene synthases, in tomato glands neryl diphosphate serves as a precursor for the synthesis of monoterpenes.