Vaccination with FimH adhesin protects cynomolgus monkeys from colonization and infection by uropathogenic Escherichia coli
Vaccination with FimH adhesin protects cynomolgus monkeys from colonization and infection by uropathogenic Escherichia coli
复制标题
DOI：
10.1086/315258
复制
发表时间：
2000-02-01
影响因子：
6.4
通讯作者：
Koenig, S
中科院分区：
文献类型：
--
作者：
Langermann, S;Möllby, R;Koenig, S
大肠杆菌FimH粘附素介导与膀胱粘膜的结合。在小鼠中，FimH疫苗可保护免受细菌攻击。在该研究中，用100 μ g与MF 59佐剂混合的FimCH粘附素-伴侣蛋白复合物接种4只猴子，并且仅肌肉内给予4只猴子佐剂。2次给药后（第0天和第4周），48周时的加强免疫在接种猴中引发了对FimH的强烈IgG抗体应答。所有8只猴均用1 mL 10（8）E攻毒。大肠杆菌膀胱炎分离株NU 14。4只接种猴中有3只未发生脓尿和脓尿;所有对照猴均被感染。这些发现表明，基于E.大肠杆菌1型药丸可能有助于预防人类膀胱炎。
Escherichia coli FimH adhesin mediates binding to the bladder mucosa. In mice, a FimH vaccine protects against bacterial challenge. In this study, 4 monkeys were inoculated with 100 mu g of FimCH adhesin-chaperone complex mixed with MF59 adjuvant, and 4 monkeys were given adjuvant only intramuscularly. After 2 doses (day 0 and week 4), a booster at 48 weeks elicited a strong IgG antibody response to FimH in the vaccinated monkeys, All 8 monkeys were challenged with 1 mL of 10(8) E. coli cystitis isolate NU14. Three of the 4 vaccinated monkeys were protected from bacteruria and pyuria; all control monkeys were infected. These findings suggest that a vaccine based on the FimH adhesin of E. coli type 1 pill may have utility in preventing cystitis in humans.