Effects of microencapsulated Enterococcus fecalis CG1.0007 on growth performance, antioxidation activity, and intestinal microbiota in broiler chickens
Effects of microencapsulated Enterococcus fecalis CG1.0007 on growth performance, antioxidation activity, and intestinal microbiota in broiler chickens
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DOI：
10.2527/jas.2012-5956
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发表时间：
2013-09-01
影响因子：
3.3
通讯作者：
Zhao, Y. X.
中科院分区：
文献类型：
--
作者：
Han, W.;Zhang, X. L.;Zhao, Y. X.
我们进行了一系列试验，以评估膳食补充剂微囊粪肠球菌 CG1.0007 对 Arbor Acres 肉鸡（“肉鸡”）生长性能、抗氧化活性和肠道微生物群的影响。总共 150 只 1 日龄肉鸡被随机分配到 5 个饲养处理（对照组饲喂基础日粮，3 组饲喂基础日粮加不同浓度的微囊 CG1.0007，1 组饲喂基础日粮加抗生素）。使用基于 16S rRNA 基因的 PCR 变性梯度凝胶电泳 (DGGE) 分析和粪便样本的实时定量 PCR 分析来研究肠道细菌重要属的变化。在42天的实验期间，与对照组相比，饲喂高浓度和中浓度微胶囊的鸡的日增重显着更高（分别为9.90％和9.50％），饲料与增益的比率显着低于对照组（分别为4.40％和4.00％）。微胶囊处理组的总抗氧化能力以及丙二醛和超氧化物歧化酶的含量在抗氧化方面表现出显着的变化。微胶囊处理组中乳酸菌和双歧杆菌的数量显着高于对照组。聚类分析表明，DGGE 细菌谱与喂养处理相关，并揭示了与微胶囊补充相关的肠道微生物群的多样性和丰富度。总之，我们的结果表明，日粮中添加微囊粪肠球菌 CG1.0007 可以增强肉鸡的生长性能并改善其健康状况。
We performed a series of trials to assess the effect of dietary supplementation with microencapsulated Enterococcus fecalis CG1.0007 on growth performance, antioxidation activity, and intestinal microbiota in Arbor Acres broiler chickens ("broilers"). A total of 150 1-d-old broilers were assigned randomly to 5 feeding treatments (a control group fed the basal diet, 3 groups fed the basal diet plus various concentrations of microencapsulated CG1.0007, and 1 group fed the basal diet plus an antibiotic). Changes in important genera of intestinal bacteria were studied using 16S rRNA gene-based PCR-denaturing gradient gel electrophoresis (DGGE) profiling and real-time quantitative PCR analysis of fecal samples. During the course of the 42-d experimental period, ADG of the birds fed the high and intermediate concentrations of microcapsules were significantly greater (9.90 and 9.50%, respectively) and the ratios of feed to gain fed were significantly lower (4.40 and 4.00%, respectively) compared with the control group. The total antioxidant capacity and the content of malondialdehyde and superoxide dismutase in the microcapsule-treated groups showed significant changes in terms of antioxidation. The numbers of Lactobacillus and Bifidobacterium were significantly greater in the microcapsule-treated groups than in the control group. Cluster analysis indicated that the DGGE bacterial profiles were related to the feeding treatments and revealing the diversity and richness of the intestinal microbiota associated with supplementation of microcapsules. In summary, our results indicate that dietary addition of microencapsulated E. fecalis CG1.0007 enhanced the growth performance of the broilers and improved their health.