Macrophage phenotype in the epigallocatechin-3-gallate (EGCG)-modified collagen determines foreign body reaction
Macrophage phenotype in the epigallocatechin-3-gallate (EGCG)-modified collagen determines foreign body reaction
复制标题
表没食子儿茶素 — 3 — 没食子酸酯 (EGCG) — 修饰胶原蛋白中的巨噬细胞表型决定了异物反应
DOI：
10.1002/term.2687
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发表时间：
2018-06-01
影响因子：
3.3
通讯作者：
Qu, Yili
中科院分区：
文献类型：
--
作者：
Chu, Chenyu;Liu, Li;Qu, Yili
胶原已被广泛应用于引导性骨再生，胶原膜的植入会引起异物反应（FBR）。FBR的不平衡常常导致牙种植失败。因此，胶原膜植入后FBR的调节变得越来越重要。在FBR中起关键作用的巨噬细胞已被区分为促炎（M1）和抗炎（M2）表型。表没食子儿茶素没食子酸酯（EGCG）修饰的胶原蛋白膜先前已被证明可调节炎症因子的分泌。在这项研究中，CD 31的免疫组织化学显示，与纯胶原膜处理的血管相比，植入EGCG修饰的胶原膜后血管面积显著扩大。此外，苏木精-伊红染色和免疫荧光显示植入EGCG修饰的胶原膜后M2巨噬细胞数量增加。此外，定量真实的时间聚合酶链反应显示，在植入EGCG修饰的胶原膜后，CXCL 1（中性粒细胞的主要化学引诱物和炎症促进剂）的表达显著下调，而STAB 1、CCR 2、CCR 3、CCL 2和CCL 3（与M2巨噬细胞相关）的表达显著上调。从这些发现中，我们得出结论，EGCG修饰的胶原膜能够调节巨噬细胞的募集和极化，从而改善FBR。
Collagen has been widely used in guided bone regeneration, and the implantation of collagen membranes will elicit the foreign body reaction (FBR). The imbalance of FBR often leads to failure of dental implants. Therefore, modulation of the FBR after implantation of collagen membranes becomes increasingly important. Macrophages, pivotal in FBR, have been distinguished into pro‐inflammatory (M1) and anti‐inflammatory (M2) phenotypes. Epigallocatechin‐3‐gallate (EGCG)‐modified collagen membranes have been previously shown to regulate secretion of inflammatory factors. In this study, immunohistochemistry of CD31 showed that areas of blood vessels were significantly enlarged after implantation of EGCG‐modified collagen membranes compared with those treated with pure collagen membranes. Besides, haematoxylin–eosin staining and immunofluorescence showed an increased number of M2 macrophages after implantation of EGCG‐modified collagen membranes. In addition, quantitative real‐time polymerase chain reaction showed that after implantation of EGCG‐modified collagen membranes, expression of CXCL1 (predominant chemoattractants to neutrophils and inflammation promotors) was significantly downregulated, whereas expressions of STAB1, CCR2, CCR3, CCL2, and CCL3 (related to M2 macrophages) were significantly upregulated. From these findings, we conclude that EGCG‐modified collagen membranes were able to regulate the recruitment and polarization of macrophages, so that ameliorate FBR.