Sago starch as a biomass source : Raw sago starch hydrolysis by commercial enzymes
Sago starch as a biomass source : Raw sago starch hydrolysis by commercial enzymes
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西米淀粉作为生物质来源：用商业酶水解生西米淀粉
DOI：
10.1016/0960-8524(95)00132-8
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发表时间：
1996
期刊：
影响因子：
--
通讯作者：
C. Oates
中科院分区：
文献类型：
--
作者：
Wen Wang;A. Powell;C. Oates
使用商业葡糖淀粉酶-AMG（EC 3·2·1·3）、α-淀粉酶-BAN、Fungamyl和Termamyl（EC 3·2·1·1）、脱支淀粉酶-Promozyme（EC 3·2·1·41）以及它们的混合物在pH 5.0的乙酸钠缓冲液（pH 3.5）中在35°C下水解生西米淀粉和通过在乙酸钠缓冲液（pH 3.5）中在60°C下加热2小时预处理的西米淀粉。与在相同条件下测试的玉米和木薯淀粉相比，生西米淀粉是酶作用的不良底物，尽管预处理淀粉增加了水解的程度和速率。结果表明，糖化酶和α-淀粉酶对西米淀粉的水解有很强的协同作用。水解产物通过高效体积排阻色谱（HPSEC）进行了表征。水解西米淀粉的总碳水化合物浓度降低，但残留物中直链淀粉和支链淀粉的比例保持不变。
Raw sago starch and sago starch pretreated by heating at 60°C for 2 hours in sodium acetate buffer (pH 3·5) were hydrolysed using commercial glucoamylase-AMG (EC 3·2·1·3), α-amylases-BAN, Fungamyl and Termamyl (EC 3·2·1·1), debranching amylase-Promozyme (EC 3·2·1·41), and their mixtures in sodium acetate buffer, pH 5·0 at 35°C. Raw sago starch was a poor substrate for enzyme action compared to corn and tapioca starches tested under the same conditions, although pretreating the starch increased the extent and rate of hydrolysis. A strong synergism between glucoamylase and α-amylase on the hydrolysis of both untreated and pretreated sago starch was observed. The hydrolysis products were characterized by high-performance size-exclusion chromatography (HPSEC). The total carbohydrate concentration of hydrolysed sago starch decreased but the amylose and amylopectin ratios in the residues remained the same.