First detection of canine parvovirus type 2c in South America
First detection of canine parvovirus type 2c in South America
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DOI：
10.1016/j.vetmic.2007.04.028
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发表时间：
2007-09-20
影响因子：
3.3
通讯作者：
Hernandez, Martfn
中科院分区：
文献类型：
--
作者：
Perez, Ruben;Francia, Lourdes;Hernandez, Martfn
自20世纪70年代初突然出现以来，犬细小病毒2型（CPV-2）一直在通过产生新的遗传和抗原变异（CPV-2a/b/c和许多其他突变）而进化，这些变异在世界各地分布不均匀。为了制定控制这些变异传播的策略和了解病毒进化是对来自不同地理位置的野毒株进行基因分型的基础。在本文中，我们从2006年收集的乌拉圭犬临床样本中检测了25株CPV。采用聚合酶链反应（PCR）、限制性片段长度多态性（RFLP）和DNA序列分析对该病毒VP2基因片段进行了分析。在分析的25株分离株中，只有1株被鉴定为CPV-2a型，24株被鉴定为CPV-2c型，表明该型是目前在乌拉圭流行的现场CPV。这是美洲大陆首次报告CPV-2c，也是迄今为止在犬类中观察到的这种类型的最高频率。它在南美洲的出现支持了CPV-2c正在像2a/2b抗原型一样达到全球分布的假设。2007 Elsevier B.V.版权所有
Since its sudden emergence in the early 1970s, canine parvovirus type-2 (CPV-2) has been evolving through the generation of novel genetic and antigenic variants (CPV-2a/b/c and a number of additional mutations) that are unevenly distributed throughout the world. In order to develop strategies to control the spread of these variants and to understand virus evolution is fundamental to genotype field isolates from different geographic locations. In the present paper we have examined 25 isolates of CPV from clinical samples of Uruguayan dogs collected during year 2006. A fragment of the VP2 gene of the virus was analyzed using polymerase chain reaction (PCR), restriction fragment length polymorphism (RFLP) and DNA sequence analysis. Out of the 25 isolates analyzed, only one was characterized as CPV-2a and 24 were characterized as CPV-2c, indicating that this type is currently the prevalent field CPV circulating in Uruguay. This is the first report of CPV-2c in the American continent and it also represents the highest frequency of this type observed in a dog population so far. Its presence in South American supports the assumption that CPV-2c is reaching a worldwide distribution as occurred with 2a/2b antigenic types. 2007 Elsevier B.V. All rights reserved.