Cellular membrane enrichment of self-assembling D-peptides for cell surface engineering.
Cellular membrane enrichment of self-assembling D-peptides for cell surface engineering.
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DOI：
10.1021/am502250r
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发表时间：
2014-06
影响因子：
9.5
通讯作者：
Huaimin Wang;Youzhi Wang;A. Han;Yanbin Cai;Nannan Xiao;Ling Wang;D. Ding;Zhimou Yang
中科院分区：
文献类型：
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作者：
Huaimin Wang;Youzhi Wang;A. Han;Yanbin Cai;Nannan Xiao;Ling Wang;D. Ding;Zhimou Yang
我们偶尔会发现一些含有D-氨基酸的自组装肽在自组装阶段会优先富含在细胞膜中，而在未组装阶段会均匀分布在细胞质中。仅含氨基酸的自组装肽在自组装和未组装阶段均均匀分布于细胞质中。因此，含有D-氨基酸的自组装肽可以应用于含肽的细胞表面工程。更重要的是，通过将蛋白质结合肽(WRESAI的PDZ结构域结合六肽)与含有D-氨基酸的自组装肽结合，蛋白质也可以被引入细胞表面。本研究不仅为细胞表面工程提供了一种新的途径，而且突出了含有D-氨基酸的自组装肽的特殊性质及其在再生医学、药物输送和组织工程中的潜在应用。
We occasionally found that several self-assembling peptides containing D-amino acids would be preferentially enriched in cellular membranes at self-assembled stages while distributed evenly in the cytoplasma of cells at unassembled stages. Self-assembling peptides containing only Lamino acids distributed evenly in cytoplasma of cells at both self-assembled and unassembled stages. The self-assembling peptides containing D-amino acids could therefore be applied for engineering cell surface with peptides. More importantly, by integrating a protein binding peptide (a PDZ domain binding hexapeptide of WRESAI) with the self-assembling peptide containing D-amino acids, protein could also be introduced to the cell surface. This study not only provided a novel approach to engineer cell surface, but also highlighted the unusual properties and potential applications of self-assembling peptides containing D-amino acids in regenerative medicine, drug delivery, and tissue engineering.