Differential gene expression underpinning production of distinct sperm morphs in the wax moth Galleria mellonella.
Differential gene expression underpinning production of distinct sperm morphs in the wax moth Galleria mellonella.
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差异基因表达支撑蜡蛾大蜡螟中不同精子形态的产生。
DOI：
10.1101/2023.12.13.571524
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发表时间：
2023
期刊：
影响因子：
--
通讯作者：
Moth E
中科院分区：
文献类型：
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作者：
Moth E
雄性鳞翅目产生两种不同类型的精子；每次射精都含有能使卵子受精的正炔精子和不能使卵子受精的非炔精子。这些精子具有不同的形态、独特的功能和不同的蛋白质组。它们的产生受到高度调控，然而，很少有基因在一种或另一种形态的产生中具有特定的作用。我们提出了第一个对正炔和无炔精子前体的比较转录组学研究，以确定可能涉及调节或制定不同分化程序的基因。差异表达基因包括在转录调控、细胞周期和精子形态中发挥潜在作用的基因。我们发现基因复制产生的同源物与功能仅限于一个或其他形态。然而，系统发育分析也揭示了不同鳞翅目物种间重复基因表达模式的进化灵活性。提高对鳞翅目昆虫繁殖的了解，对于防治农业中普遍存在的害虫至关重要，而另一方面，也可以确保传粉昆虫种群在环境压力下的繁殖力和生存。
Male Lepidoptera produce two distinct sperm types; each ejaculate contains both eupyrene sperm, which can fertilize the egg, and apyrene sperm, which are not fertilization competent. These sperm have distinct morphologies, unique functions and different proteomes. Their production is highly regulated, however, very few genes with specific roles in the production of one or other morph have been described. We present the first comparative transcriptomics study of precursors of eupyrene and apyrene sperm to identify genes potentially implicated in regulating or enacting the distinct differentiation programmes. Differentially expressed genes included genes with potential roles in transcriptional regulation, cell cycle and sperm morphology. We identified gene duplications generating paralogues with functions restricted to one or other morph. However, phylogenetic analysis also revealed evolutionary flexibility in expression patterns of duplicated genes between different lepidopteran species. An improved understanding of lepidopteran reproduction will be vital in targeting prevalent pests in agriculture, and on the flip side, ensuring the fertility and thus survival of pollinator populations in response to environmental stress.