Selective laser sintered nano-HA/PDLLA composite microspheres for bone scaffolds applications
Selective laser sintered nano-HA/PDLLA composite microspheres for bone scaffolds applications
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DOI：
10.1108/rpj-06-2019-0155
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发表时间：
2020-05-29
影响因子：
3.9
通讯作者：
Shi, Yusheng
中科院分区：
文献类型：
--
作者：
Lin, Kesheng;Liu, Jie;Shi, Yusheng
目的体内植入后无菌炎症的主要原因是聚L-丙交酯(PLLA)和聚D-丙交酯(PLLA)的降解吸收速率较慢，而聚D，L-丙交酯(PDLLA)由于其无定形结构，其降解速度比PLLA快得多。此外，羟基磷灰石(HA)的水解物是碱性的，可以中和聚乳酸水解引起的局部组织过酸。设计/方法/途径本研究选择选择性激光烧结技术，以水包油(S/O/W)法制备纳米HA/聚乳酸复合微球。首先由单层实验结果确定了块材的SLS参数范围，然后通过正交实验得到了优化的参数。对优化后的SLS样品的拉伸性能、疏水性、生物相容性、生物毒性和体外降解性进行了表征，结果表明，由于样品内部孔洞较多，拉伸强度较低，有利于更好的细胞粘附性和营养物质的转运。另外，纳米羟基磷灰石的加入使样品的疏水性得到了提高，但纳米羟基磷灰石保留了其固有性能。此外，样品与大量细胞通过伪足附着在材料上表现出良好的生物相容性，纯PDLLA和10%HA/PDLLA的生物毒性没有显著差异。本研究将S/光/水技术与激光烧结技术相结合，为进一步利用纳米羟基磷灰石/聚乳酸复合材料制备载药微球支架提供了理论基础。
PurposeThe main cause of aseptic inflammation after an in vivo implantation is that Poly(L-lactide) (PLLA) and Poly(D-lactide) have a slower degradation and absorption rate, while Poly(D, L-lactide) (PDLLA) has a much faster degradation rate than PLLA because of its amorphous structure. Also, the hydrolyzate of Hydroxyapatite (HA) is alkaline, which can neutralize local tissue peracid caused by hydrolysis of Polylactic acid.Design/methodology/approachIn this study, the selective laser sintering (SLS) technique was chosen to prepare bone scaffolds using nano-HA/PDLLA composite microspheres, which were prepared by the solid-in-oil-in-water (S/O/W) method. First, the SLS parameters range of bulk was determined by the result of a single-layer experiment and the optimized parameters were then obtained by the orthogonal experiment. The tensile property, hydrophobicity, biocompatibility, biological toxicity and in vitro degradation of the samples with optimized SLS parameters were characterized.FindingsAs a result, the samples showed a lower tensile strength because of the many holes in their interior, which was conducive to better cell adhesion and nutrient transport. In addition, the samples retained their inherent properties after SLS and the hydrophobicity was improved after adding nano-HA because of the OH group. Furthermore, the samples showed good biocompatibility with the large number of cells adhering to the material through pseudopods and there was no significant difference between the pure PDLLA and 10% HA/PDLLA in terms of biological toxicity. Finally, the degradation rate of the composites could be tailored by the amount of nano-HA.Originality/valueThis study combined the S/O/W and SLS technique and provides a theoretical future basis for the preparation of drug-loaded microsphere scaffolds through SLS using HA/PDLLA composites.