APC/CCDC20 targets SCFFBL17 to activate replication stress responses in Arabidopsis
APC/CCDC20 targets SCFFBL17 to activate replication stress responses in Arabidopsis
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DOI：
10.1093/plcell/koac360
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发表时间：
2022-12-12
期刊：
影响因子：
11.6
通讯作者：
Yan，Shunping
中科院分区：
文献类型：
--
作者：
Pan，Ting;Gao，Shan;Yan，Shunping
DNA复制胁迫威胁着基因组的稳定性，影响植物的生长发育。植物是如何解决复制压力的，人们对此知之甚少。WEE1介导的细胞周期停滞是复制应激反应所必需的。E3泛素连接酶后期促进复合体/环体(APC/C)和Skp1/cullin 1/F-box(SCF)是细胞周期的重要调节因子。在这里，我们证明了APC/CCDC20在拟南芥的复制胁迫反应中介导了SCFFBL17的降解。在生物化学方面，WEE1与APC/C共激活子APC10相互作用并磷酸化，APC10增强F-box-LIKE17(FBL17)与细胞分裂周期20(CDC20)之间的相互作用，CDC20是APC/C的激活剂。从基因上讲，沉默CDC20或APC10使植物对复制胁迫敏感，而FBL17的缺失抑制了复制胁迫。综上所述，我们的研究表明，WEE1激活APC/C来抑制FBL17，从而为植物的复制胁迫反应提供了洞察。
DNA replication stress threatens genome stability and affects plant growth and development. How plants resolve replication stress is poorly understood. The protein kinase WEE1-mediated cell cycle arrest is required for replication stress responses. The E3 ubiquitin ligases anaphase-promoting complex/cyclosome (APC/C) and Skp1/Cullin 1/F-box (SCF) are essential regulators of the cell cycle. Here, we show that APC/CCDC20mediates the degradation of SCFFBL17during replication stress responses inArabidopsis thaliana. Biochemically, WEE1 interacts with and phosphorylates the APC/C co-activator APC10, which enhances the interaction between F-BOX-LIKE17 (FBL17) and CELL DIVISION CYCLE 20 (CDC20), an activator of APC/C. Both APC10 and CDC20 are required for the polyubiquitination and degradation of FBL17. Genetically, silencingCDC20orAPC10confers plant hypersensitivity to replication stress, which is suppressed by loss ofFBL17. Collectively, our study suggests that WEE1 activates APC/C to inhibit FBL17, providing insight into replication stress responses in plants.