TRANSFORMING GROWTH FACTOR-BETA(1)-INDUCED EXPRESSION OF THE MUCOSA-RELATED INTEGRIN ALPHA(E) ON LYMPHOCYTES IS NOT ASSOCIATED WITH MUCOSE-SPECIFIC HOMING
TRANSFORMING GROWTH FACTOR-BETA(1)-INDUCED EXPRESSION OF THE MUCOSA-RELATED INTEGRIN ALPHA(E) ON LYMPHOCYTES IS NOT ASSOCIATED WITH MUCOSE-SPECIFIC HOMING
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DOI：
10.1002/eji.1830250602
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发表时间：
1995-06-01
影响因子：
5.4
通讯作者：
HAMANN, A
中科院分区：
文献类型：
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作者：
AUSTRUP, F;REBSTOCK, S;HAMANN, A
整合素α（E）（HML-1，α（IEL），α（M290））主要在上皮部位，特别是肠粘膜的淋巴细胞上表达。我们研究了α（E）是否在归巢中有任何作用或描绘出具有不同迁移行为的表型。在存在或不存在转化生长因子（TGF）β（1）的情况下，用抗CD 3刺激淋巴结T细胞5天，分别产生α（E）（+）或α（E）（-）细胞。然后在小鼠中测试这两个种群的归巢特性。CD 4（+）或CD 8（+）亚群的α（E）（+）（TGF-β处理）和α（E）（-）（对照）细胞进入肠道的能力较低，并对各种其他器官表现出相同的归巢行为。同样的情况也适用于α（E）（+）和α（E）（-）细胞，这些细胞用抗CD 3短暂刺激（24 h），然后在注射前恢复到静息状态，尽管在这种情况下，两种细胞群通过淋巴组织再循环的能力比完全激活的鳗鱼更强。结果表明，α（E）β（7）不作为归巢受体，并且位点特异性标记α（E）的表达与独特的归巢行为无关。
The integrin alpha(E) (HML-1, alpha(IEL), alpha(M290)) is largely expressed on lymphocytes in epithelial sites, especially the gut mucosa. We investigated whether alpha(E) has any role in homing or delineates a phenotype with distinct migratory behavior. Lymph node T cells were stimulated for 5 days with anti-CD3 in the presence or absence of transforming growth factor (TGF)beta(1), to generate alpha(E)(+) or alpha(E)(-) cells, respectively. The two populations were then tested for their homing properties in mice. Both alpha(E)(+) (TGF-beta-treated) and alpha(E)(-) (control) cells of either CD4(+) or CD8(+) subset had a low capacity to enter the gut and showed the same homing behavior with respect to a variety of other organs. The same was true for alpha(E)(+) and alpha(E)(-) cells that had been briefly stimulated with anti-CD3 (24 h) and then allowed to return to a resting state before injection, though in this case both populations showed a greater capacity to recirculate through lymphoid tissue than was seen with fully activated eels. The results indicate that alpha(E) beta(7) does not act as a homing receptor, and that the expression of the site-specific marker alpha(E) does not correlate with a distinct homing behavior.