REGIONAL DIFFERENCES IN QUANTITIES OF HISTOCHEMICALLY DETECTABLE MUCOSUBSTANCES IN NASAL, PARA-NASAL, AND NASOPHARYNGEAL EPITHELIUM OF THE BONNET MONKEY
REGIONAL DIFFERENCES IN QUANTITIES OF HISTOCHEMICALLY DETECTABLE MUCOSUBSTANCES IN NASAL, PARA-NASAL, AND NASOPHARYNGEAL EPITHELIUM OF THE BONNET MONKEY
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DOI：
10.1177/35.3.2434556
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发表时间：
1987-03-01
影响因子：
3.2
通讯作者：
STGEORGE, JA
中科院分区：
文献类型：
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作者：
HARKEMA, JR;PLOPPER, CG;STGEORGE, JA
吸入刺激物可诱导动物鼻上皮细胞增生。要用组织化学方法描述这种反应，首先要了解正常鼻腔上皮粘液物质的组织化学特征和分布。一种自动化的图像分析方法被用来检测和定量酸性，中性和硫酸化的粘液物质在上皮内衬的鼻和鼻旁气道的8个帽猴。将来自这些气道的限定区域的2 μ m厚的组织切片用阿尔新蓝/高碘酸-希夫染色以显示酸性和中性粘液物质，或用高铁二胺染色以显示硫酸化粘蛋白。覆盖上鼻甲的呼吸道上皮细胞每单位基底膜表面积的粘液物质体积最大，而上颌窦上皮细胞最少。沿鼻腔的中隔壁和侧壁沿着的总上皮粘液物质的数量一般呈前后增加，并且后鼻气道中的酸性粘液物质多于中性粘液物质。上颌窦上皮粘液物质以中性为主。因此，我们的结论是，有大量的区域定量差异，在灵长类鼻上皮细胞，必须考虑鼻粘膜刺激诱导的上皮粘蛋白的变化时，在鼻粘膜中的粘液物质。
Inhaled irritants induce secretory cell hyperplasia in nasal epithelium of animals. To characterize this response histochemically it is first important to know the histochemical character and distribution of epithelial mucosubstance in the normal nasal cavity. An automated image analyzing method was used to detect and quantitate acidic, neutral, and sulfated mucosubstances in the epithelium lining the nasal and paranasal airways of eight bonnet monkeys. Tissue sections 2 .mu.m thick from defined regions of these airways were stained with either alcian blue/periodic acid-Schiff to demonstrate acid and neutral mucosubstances or high iron diamine to demonstrate sulfated mucins. Respiratory epithelium covering maxilloturbinates had the largest volume of stainable mucosubstance per unit surface area of basal lamina, whereas the maxillary sinus epithelium had the least. There was a general anteroposterior increase in the quantity of total epithelial mucosubstance along the septal and lateral walls of the nasal cavity, and there was more acidic than neutral mucosubstance in the posterior nasal airway than in the anterior. Epithelial mucosubstance in the maxillary sinus was predominantly neutral. Therefore, we conclude that there are substantial regional quantitative differences in stainable mucosubstances in the primate nasal epithelium which must be considered when examining nasal mucosa for irritant-induced changes in epithelial mucins.