Fabrication of Enzyme-Loaded Cartridges Using CO2-Assisted Polymer Compression

Fabrication of Enzyme-Loaded Cartridges Using CO2-Assisted Polymer Compression
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DOI:
10.3390/technologies9040085
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发表时间:
2021-12-01
期刊:
影响因子:
3.6
通讯作者:
Matsuura, Shun-ichi
Matsuura, Shun-ichi
中科院分区:
其他
文献类型:
--
作者:
Aizawa, Takafumi;Matsuura, Shun-ichi

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使用CO2辅助聚合物压缩(CAPC)制造装载酶的盘形药筒,CAPC是一种不使用热量的聚合物粘合方法。在该过程中,在将酶装载到介孔二氧化硅上之后,将其放置在使用CAPC由层压纤维片制成的容器中。随后,盖和容器通过CAPC粘合。使用偶氮染料(甲基红)的还原分解反应的验证表明,酶没有失活,并且成功地创建了反应盒。
An enzyme-loaded disk-shaped cartridge was fabricated using CO2-assisted polymer compression (CAPC), which is a polymer-bonding method that does not use heat. In this process, after the enzyme was loaded onto mesoporous silica, it was placed in a container fabricated from laminated fibrous sheets using CAPC. Subsequently, the lid and container were bonded via CAPC. Verification using the reductive decomposition reaction of an azo dye (methyl red) showed that the enzyme was not deactivated and that a reaction cartridge was created successfully.