Salivary Gland Protein Repertoire from Aedes aegypti Mosquitoes
Salivary Gland Protein Repertoire from Aedes aegypti Mosquitoes
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DOI：
10.1089/vbz.2009.0042
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发表时间：
2010-05-01
影响因子：
2.1
通讯作者：
Rogier, Christophe
中科院分区：
文献类型：
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作者：
Almeras, Lionel;Fontaine, Albin;Rogier, Christophe
由节肢动物传播的病毒引起的疾病是对世界各地人类和动物种群健康的重大威胁。更好地了解嗜血节肢动物唾液腺和唾液中合成的分子可能有助于改善病原体传播的控制。最近，我们在实验室对埃及伊蚊的三个种群(Paea、Rockefeller和Formosus)进行了唾液组分析，从而鉴定出44种唾液蛋白。在这些分泌蛋白中，没有一种是在一个群体中唯一表达的，这表明唾液蛋白的表达在不同群体中高度保守。在另一项研究中，我们报告了其中一些唾液蛋白可以作为旅行者接触蚊媒的属特异性标记。在这里，比较这三个相同的Ae之间的唾液腺蛋白图谱。采用一维十二烷基硫酸钠-聚丙烯酰胺凝胶电泳法(SDS-PAGE)差示凝胶电泳法进行埃及伊蚊菌落鉴定。在唾液水平上观察到，这三个菌落之间没有显著差异。来自Ae.的唾液腺蛋白质谱。用蛋白质组学方法对埃及蚊进行了分析。在这些唾液腺中发现了120种蛋白质，这是对Ae的最大描述。埃及伊蚊唾液腺蛋白质目录。我们成功地鉴定了15种分泌蛋白，其中一些已经被报道与血液摄取有关。对唾液腺和唾液腺的蛋白质进行了比较。
Diseases caused by arthropod-borne viruses are a significant threat to the health of human and animal populations throughout the world. Better knowledge of the molecules synthesized in the salivary gland and saliva of hematophagous arthropods could be of use for improving the control of pathogen transmission. Recently, a sialome analysis of three Aedes aegypti mosquito colonies (PAEA, Rockefeller, and Formosus) carried out in our laboratory allowed us to identify 44 saliva proteins. Of these secreted proteins, none was exclusively expressed in one colony, suggesting that expression of salivary proteins is highly conserved across populations. In another study, we reported that some of these salivary proteins could be used as the genus-specific markers for travelers' exposure to mosquito vectors. Here, comparison of salivary gland protein profiles between these same three Ae. aegypti colonies was performed using the one-dimensional sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) difference gel electrophoresis method. As observed at the saliva level, no significant differences were detected between these three colonies. The salivary gland protein repertoire from the Ae. aegypti mosquito was analyzed using a proteomic approach. One hundred and twenty proteins were identified in these salivary glands representing the largest description of the Ae. aegypti salivary gland protein catalog. We succeeded in identifying 15 secreted proteins, some of which have already been reported as being involved in blood feeding. A comparison of the proteins identified between the salivary glands and the sialome is discussed.